Characterization of Novel Bacteriophage AhyVDH1 and Its Lytic Activity Against Aeromonas hydrophila
Characterization of Novel Bacteriophage AhyVDH1 and Its Lytic Activity Against Aeromonas hydrophila
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新型噬菌体 AhyVDH1 的表征及其对嗜水气单胞菌的裂解活性
DOI：
10.1007/s00284-020-02279-7
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发表时间：
2020-11-11
影响因子：
2.6
通讯作者：
Xiao, Wei
中科院分区：
文献类型：
--
作者：
Cheng, Yahui;Gao, Dongye;Xiao, Wei
噬菌体疗法是克服多重耐药细菌问题的另一种方法。本研究分离了一种感染嗜水气单胞菌 4572 的新型噬菌体 AhyVDH1，并对其形态、一步生长曲线、裂解活性、各种条件下的稳定性和基因组进行了研究。透射电镜显示AhyVDH1具有直径49 nm的二十面体头部和长度127 nm的收缩尾部，表明它属于肌病毒科。 AhyVDH1 对 A. Hydrophila 4572 的表面表现出强烈的吸附作用（10 分钟内吸附 90%）。 AhyVDH1的潜伏期为50分钟，爆发大小为274个空斑形成单位/感染细胞。 AhyVDH1在30℃下稳定1小时，在60℃下加热20分钟后失去感染性。在 pH 6-7 1 小时内感染性不受影响，而噬菌体在 pH < 4 或 > 11 时失活。 AhyVDH1 基因组长 39,175 bp，G + C 含量为 58%，开放阅读框 59 个。 BLAST分析表明噬菌体AhyVDH1的基因组序列与气单胞菌噬菌体Ahp2的基因组序列相关。 AhyVDH1 观察到时间和 MOI 依赖性的体外嗜水放线菌生长抑制。治疗后约 12 小时出现宿主细菌的重新生长，表明其在治疗嗜水放线菌感染方面具有潜在的治疗价值，但应开发噬菌体混合物。
Phage therapy is an alternative approach to overcome the problem of multidrug-resistant bacteria. Here, a novel bacteriophage AhyVDH1, which infects Aeromonas hydrophila 4572, was isolated and its morphology, one-step growth curve, lytic activity, stability under various conditions, and genome were investigated. Transmission electron microscopy revealed that AhyVDH1 has an icosahedral head 49 nm in diameter and a contractile tail 127 nm in length, suggesting that it belongs to the family Myoviridae. AhyVDH1 showed strong adsorption to the surface of A. hydrophila 4572 (90% in 10 min). The latent period of AhyVDH1 was shown to be 50 min, and the burst size was 274 plaque-forming unit/infected cell. AhyVDH1 was stable at 30 °C for 1 h and lost infectivity after20 min of heating at 60 °C. Infectivity remained unaffected at pH 6-7 for 1 h, while the bacteriophage was inactivated at pH < 4 or > 11. AhyVDH1 has a 39,175-bp genome, with a 58% G + C content and 59 open reading frames. BLAST analysis indicated that the genome sequence of phage AhyVDH1 was related to that of Aeromonas phage Ahp2. Both time and MOI-dependent in vitro A. hydrophila growth inhibition were observed with AhyVDH1.Re-growth of the host bacteria appeared about 12 h after treatment, suggesting its potential therapeutic value in treating A. hydrophila infections, but phage cocktails should be developed.