PARAVENTRICULAR NUCLEUS REGION CONTROLS PITUITARY-ADRENAL-FUNCTION IN BRATTLEBORO RATS
PARAVENTRICULAR NUCLEUS REGION CONTROLS PITUITARY-ADRENAL-FUNCTION IN BRATTLEBORO RATS
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DOI：
10.1152/ajpregu.1983.244.3.r363
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发表时间：
1983-01-01
影响因子：
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通讯作者：
MAKARA, GB
中科院分区：
文献类型：
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作者：
BAERTSCHI, AJ;BENY, JL;MAKARA, GB
为研究室旁核和神经垂体激素在调节ACTH分泌中的作用，用刀损毁Brattleboro尿崩症大鼠（DI）的室旁核（PV），假损毁DI（S）作为对照。4天后，大鼠通过吸入应激，并在应激期间和应激后30-40 min采集血样用于测定皮质酮。将正中隆起（ME）和神经叶（NL）在50 μ l的0.1N HCl中匀浆并冷冻，等待促肾上腺皮质激素释放因子（CRF）的生物测定。肺静脉损害几乎消除了皮质酮分泌乙醚和减少MECRF含量3至7倍。S中NL-CRF含量约为ME的2倍，催产素占NL-CRF的60%以上。PV损伤对NL CRF活性无影响。低剂量催产素（2 mU/ml）对L.显然，PV是DI中CRF合成或CRF纤维转运的最重要部位之一;皮质酮分泌到乙醚应激主要由ME CRF控制;并且大部分CRF纤维到NL可能起源于PV外。
To study the role of the paraventricular nucleus and of neurohypophysial hormones in the control of ACTH secretion, the paraventricular nuclei (PV) of Brattleboro diabetes insipidus rats (DI) were lesioned (L) with a knife; sham-lesioned DI (S) served as controls. Four days later, the rats were stressed by either inhalation, and blood samples were taken during and 30-40 min after stress for the determination of corticosterone. The median eminence (ME) and neural lobe (NL) were homogenized in 50 .mu.l of 0.1 N HCl and frozen pending bioassay of corticotropin-releasing factor (CRF). PV lesion almost abolished the corticosterone secretion to ether and reduced the ME CRF content 3- to 7-fold. The NL CRF content in S was about twice that of ME, and oxytocin accounted for more than 60% of NL CRF. PV lesion had no effect on NL CRF activity. Low amounts of oxytocin (2 mU/ml) had no significant CRF activity but potentiated the ME CRF effect in L. Apparently PV is one of the most important sites for CRF synthesis or CRF fiber transit in DI; corticosterone secretion to ether stress is governed mainly by ME CRF; and a large proportion of CRF fibers to NL probably originates outside PV.