Protein phosphatase-type 2B is involved in the regulation of the acrosome reaction but not in the temperature-dependent flagellar movement of fowl spermatozoa
Protein phosphatase-type 2B is involved in the regulation of the acrosome reaction but not in the temperature-dependent flagellar movement of fowl spermatozoa
复制标题
DOI：
10.1530/rep.1.00327
复制
发表时间：
2004-12-01
期刊：
影响因子：
3.8
通讯作者：
Tsuzuki, Y
中科院分区：
文献类型：
--
作者：
Ashizawa, K;Wishart, GJ;Tsuzuki, Y
鸡精子在TES/NaCl缓冲液中的活力和顶体完整性在40 ℃时几乎可以忽略不计，无论是否有从产蛋的鸡卵制备的均质化内卵周层（IPVL）。然而，即使在40 ℃时，当加入2 mmol CaCl 2/l时，运动也变得活跃，并且顶体反应在IPVL存在时也被刺激，但在IPVL不存在时则不被刺激。溴氰菊酯或氰戊菊酯，蛋白磷酸酶213型（PP 2B）的特异性抑制剂的存在下，不允许在40 ℃下恢复运动性，但在IPVL的存在下，这些化合物刺激顶体反应，在1-1000 nmol/l的范围内呈剂量依赖性。这些结果表明，IPVL是激活家禽精子顶体反应所必需的，而Ca 2+在刺激精子运动和顶体胞吐中起着重要作用。此外，它似乎是调节家禽精子的顶体反应的细胞内分子机制是不同的运动力的恢复，即蛋白质去磷酸化的PP 2B在前者，而不是在后者的情况下。
The motility and acrosomal integrity of fowl spermatozoa in TES/NaCl buffer, with or without homogenized inner perivitelline layers (IPVL) prepared from laid fowl eggs, was almost negligible at 40degreesC. However, motility became vigorous even at 40degreesC when 2 mmol CaCl2/l was added, and the acrosome reaction was also stimulated in the presence, but not in the absence, of IPVL. The presence of deltamethrin or fenvalerate, specific inhibitors of protein phosphatase-type 213 (PP2B), did not permit the restoration of motility at 40degreesC but, in the presence of IPVL, these compounds stimulated the acrosome reaction in a dose-dependent manner in the range of 1-1000 nmol/l. These results suggest that IPVL is necessary for the activation of the acrosome reaction in fowl spermatozoa and that Ca2+ plays an important role in the stimulation of motility and acrosomal exocytosis. Furthermore, it appears that the intracellular molecular mechanisms for the regulation of the acrosome reaction of fowl spermatozoa are different from those for the restoration of motility, i.e. protein dephosphorylation by PP2B in the former but not in the latter case.