Gene flow between chromosomal forms of the malaria vector Anopheles funestus in Cameroon, Central Africa, and its relevance in malaria fighting
Gene flow between chromosomal forms of the malaria vector Anopheles funestus in Cameroon, Central Africa, and its relevance in malaria fighting
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DOI：
10.1534/genetics.103.025031
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Fontenille, D
中科院分区：
文献类型：
--
作者：
Cohuet, A;Dia, I;Fontenille, D
对主要媒介物种的种群结构的了解是了解疟疾流行病学的基础，在正在制定的遗传控制战略的背景下变得至关重要。尽管非洲主要疟疾媒介福氏按蚊在流行病学上具有重要意义，但与冈比亚按蚊复合体成员相比，它受到的关注要少得多。之前的染色体数据显示，西非的种群内部存在高度的结构，并导致了两种染色体形式的特征，即“Kiriina”和“Folonzo”。在中非，几乎没有可用的数据。因此，我们对AN的遗传结构进行了评估。喀麦隆的Funestus种群提高了染色体倒位和微卫星标记。微卫星标记显示，在每个本地种群中，没有特别偏离全混血的情况，并且遗传结构与距离隔离一致。然而，细胞遗传学研究表明，无论是在种群内还是在种群之间，染色体的异质性都很高。染色体倒位的分布不是随机的，根据生态条件观察到频率的倾斜性。过渡区某些地方异核型严重缺失，表明中国按蚊在染色体形式上有进一步的细分。一种。位于倒位断裂点内的Funestus微卫星遗传标记在种群中不存在分化，而在An.冈比亚倒位会影响标记基因座上的基因流动。这些结果与通过将转基因引入媒介种群来控制疟疾的战略有关。
Knowledge of population structure in a major vector species is fundamental to an understanding of malaria epidemiology and becomes crucial in the context of genetic control strategies that are being dcvelopcd. Despite its epidemiological importance, the major African malaria vector Anopheles funestus has received far less attention than members of the Anopheles gambiae complex. Previous chromosomal data have shown a high degree of structuring within populations from West Africa and have led to the characterization of two chromosomal forms, "Kiribina" and "Folonzo." In Central Africa, few data were available. We thus undertook assessment of genetic structure of An. funestus populations from Cameroon rising chromosomal inversions and microsatellite markers. Microsatellite markers revealed no particular departure from panmixia within each local population and a genetic structure consistent with isolation by distance. However, cytogenetic studies demonstrated high levels of chromosomal heterogeneity, both within and between populations. Distribution of chromosomal inversions was not random and a cline of frequency was observed, according to ecotypic conditions. Strong deficiency of heterokaryotypes was found in certain localities in the transition area, indicating a subdivision of An.funestus in chromosomal forms. An. funestus microsatellite genetic markers located within the breakpoints of inversions are not differentiated in populations, whereas in An. gambiae inversions can affect gene flow at marker loci. These results are relevant to strategies for control of malaria by introduction of transgenes into populations of vectors.