Differential expression of the noradrenaline transporter in adrenergic chromaffin cells, ganglion cells and nerve fibres of the rat adrenal medulla
Differential expression of the noradrenaline transporter in adrenergic chromaffin cells, ganglion cells and nerve fibres of the rat adrenal medulla
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DOI：
10.1016/s0891-0618(00)00113-7
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发表时间：
2001-01-01
影响因子：
2.8
通讯作者：
Lipski, J
中科院分区：
文献类型：
--
作者：
Phillips, JK;Dubey, R;Lipski, J
通过免疫组织化学和共聚焦显微镜检查，在大鼠肾上腺髓质的各种细胞和纤维群中鉴定出去甲肾上腺素转运蛋白（NAT）的表达。所有嗜铬细胞中均存在儿茶酚胺生物合成酶酪氨酸羟化酶 (TH)、芳香族 L-氨基酸脱羧酶 (AADC) 和多巴胺 β-羟化酶 (DBH) 的免疫反应性，而苯乙醇胺 N-甲基转移酶 (PNMT) 用于确定肾上腺素能嗜铬细胞组。 NAT 抗体标记主要在细胞质中，并与 PNMT 免疫反应性共定位。去甲肾上腺素能嗜铬细胞不具有 NAT 免疫反应性。此外，NAT 抗体标记显示了神经节细胞（推测为 I 型）和神经纤维簇。采用逆转录聚合酶链式反应（RT-PCR）检测肾上腺髓质穿孔和单个嗜铬细胞TH、AADC、DBH、PNMT和NAT mRNA的表达，结果与免疫细胞化学获得的结果一致。用抗生长相关蛋白 43 (GAP-43) 的抗体标记的嗜铬细胞和纤维不具有 NAT 免疫反应性，而神经节细胞则用这两种抗体双重标记。肾上腺素能嗜铬细胞中存在 NAT，而去甲肾上腺素能细胞中不存在 NAT，这表明肾上腺素能细胞类型主要负责肾上腺髓质中儿茶酚胺的摄取。 (C) 2001 Elsevier Science B.V. 保留所有权利。
Expression of the noradrenaline transporter (NAT) was identified in various cell and fibre populations of the rat adrenal medulla, examined with immunohistochemistry and confocal microscopy. Immunoreactivity for the catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), aromatic-L-amino-acid decarboxylase (AADC) and dopamine beta -hydroxylase (DBH) was present in all chromaffin cells, while phenylethanolamine N-methyltransferase (PNMT) was used to determine adrenergic chromaffin cell groups. Labelling with NAT antibody was predominantly cytoplasmic and colocalised with PNMT immunoreactivity. Noradrenergic chromaffin cells were not NAT immunoreactive. Additionally, NAT antibody labelling demonstrated clusters of ganglion cells (presumably Type I) and nerve fibres. Expression of TH, AADC, DBH, PNMT and NAT mRNA was examined using reverse transcription-polymerase chain reaction (RT-PCR) from adrenal medulla punches and single chromaffin cells, and results were consistent with those obtained with immunocytochemistry. Chromaffin cells and fibres labelled with antibodies against growth associated protein-43 (GAP-43) were not NAT immunoreactive, while ganglion cells were doubled labelled with the two antibodies. The presence of NAT in adrenergic chromaffin cells, and its absence from noradrenergic cells, suggests that the adrenergic cell type is primarily responsible for uptake of catecholamines in the adrenal medulla. (C) 2001 Elsevier Science B.V. All rights reserved.