Exosome secretion of dendritic cells is regulated by Hrs, an ESCRT-0 protein
Exosome secretion of dendritic cells is regulated by Hrs, an ESCRT-0 protein
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DOI：
10.1016/j.bbrc.2010.07.083
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发表时间：
2010-08-27
影响因子：
3.1
通讯作者：
Sugamura, Kazuo
中科院分区：
文献类型：
--
作者：
Tamai, Keiichi;Tanaka, Nobuyuki;Sugamura, Kazuo
外来体是源自抗原呈递细胞中的多泡体（MVB）的纳米囊泡。ESCRT（转运所需的内体分选复合物）途径的组分对于MVB的形成至关重要，然而ESCRT途径与外泌体分泌之间的关系仍不清楚。我们在这里证明，Hrs，一种ESCRT-0蛋白，是树突状细胞（DC）中分泌外泌体所必需的。超微结构分析显示，在对照和Hrs缺失的DC的培养上清液中存在典型的碟形外泌体。然而，外泌体分泌的量显着减少Hrs-耗尽的DC刺激后，卵清蛋白（OVA）以及钙离子载体。抗原呈递活性在从Hrs耗尽的DC纯化的外泌体中也被抑制，而在Hrs耗尽的DC中未观察到OVA降解的改变。这些数据表明Hrs通过外泌体分泌参与抗原呈递活性的调节。(C)2010年爱思唯尔公司All rights reserved.
Exosomes are nanovesicles derived from multivesicular bodies (MVBs) in antigen-presenting cells. The components of the ESCRT (endosomal sorting complex required for transport) pathway are critical for the formation of MVBs, however the relationship between the ESCRT pathway and the secretion of exosomes remains unclear. We here demonstrate that Hrs, an ESCRT-0 protein, is required for fascilitating the secretion of exosomes in dendritic cells (DCs). Ultrastructural analyses showed typical saucer-shaped exosomes in the culture supernatant from both the control and Hrs-depleted DCs. However, the amount of exosome secretion was significantly decreased in Hrs-depleted DCs following stimulations with ovalbumin (OVA) as well as calcium ionophore. Antigen-presentation activity was also suppressed in exsosomes purified from Hrs-depleted DCs, while no alteration in OVA degradation was seen in Hrs-depleted DCs. These data indicated that Hrs is involved in the regulation of antigen presentation activity through the exosome secretion. (C) 2010 Elsevier Inc. All rights reserved.