Oct-4 expression in human endometrium
Oct-4 expression in human endometrium
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DOI：
10.1093/molehr/gah254
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发表时间：
2006-01-01
影响因子：
4
通讯作者：
Kolbus, A
中科院分区：
文献类型：
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作者：
Matthai, C;Horvat, R;Kolbus, A
转录因子Oct-4对于维持细胞多能性至关重要，并且已知在胚胎干细胞、生殖细胞和发育的各个阶段的整个胚胎中表达。Oct-4以剂量依赖性方式调节细胞命运，并在生殖细胞肿瘤中发挥关键作用。在过去，几个干细胞标记物已被检测到，它们在疾病的发病机制中的作用已被频繁讨论。因此，我们研究了Oct-4的表达，比较其发生在子宫内膜的健康和患病妇女使用免疫组化（IHC）和RT-PCR。IHC显示，60张切片中有25张（42%）有Oct-4表达，25例患者中有11例（44%）有Oct-4表达。Oct-4 mRNA在所有检测的子宫内膜样本（9/9）中通过RT-PCR检测到，尽管表达水平不同。据我们所知，这是第一项证明Oct-4在人子宫内膜中表达的研究。
The transcription factor Oct-4 is crucial for the maintenance of cell pluripotency and is known to be expressed in embryonic stem cells, germ cells and whole embryos at various stages of development. Oct-4 regulates cell fate in a dose-dependent manner and plays a key role in germ-cell tumours. In the past, several stem-cell markers have been detected, and their role in the pathogenesis of diseases has been discussed frequently. Thus, we investigated the expression of Oct-4 comparing its occurrence in endometrium of healthy and diseased women using immunohistochemistry (IHC) and RT-PCR. IHC demonstrated Oct-4 expression in 25 of 60 sections (42%), respectively in 11 out of 25 patients (44%). Oct-4 mRNA was detected by RT-PCR in all tested samples (9 of 9) of endometrium, although the levels of expression varied. To our knowledge, this is the first study demonstrating Oct-4 expression in human endometrium.