Expressions of ECE-CYC2 clade genes relating to abortion of both dorsal and ventral stamens in Opithandra (Gesneriaceae).
Expressions of ECE-CYC2 clade genes relating to abortion of both dorsal and ventral stamens in Opithandra (Gesneriaceae).
复制标题
苦苣苔科背腹雄蕊败育相关的 ECE-CYC2 进化枝基因的表达
DOI：
10.1186/1471-2148-9-244
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发表时间：
2009-10-07
影响因子：
3.4
通讯作者：
Wang YZ
中科院分区：
文献类型：
--
作者：
Song CF;Lin QB;Liang RH;Wang YZ
背景
已知在金鱼草花背腹不对称（左右对称）模式中的ECE-CYC 2进化枝基因在包括抑制背雄蕊的背侧身份功能中是保守的。然而，它仍然不确定是否同样的机制的基础上的腹雄蕊，一个重要的形态特征相关的进化和多样化的左右对称的Lamiales广义，主要的主要分支左右对称开花的被子植物的退化。后蕊花属（苦苣苔科）在解决这个问题方面特别感兴趣，因为它位于唇形科的基部，这类左右对称的早期代表
结果
我们研究了4个ECE-CYC 2分支基因和两个推定的目标cyclinD 3基因在Opithandra中的表达模式，采用RNA原位杂交和RT-PCR。OpdCYC基因的表达与后雄蕊的背侧和腹侧雄蕊的败育相关，其推定的靶基因OpdcyclinD 3的负相关表达加强了这一点。OpdCYC与OpdcyclinD 3的互补表达进一步表明，OpdCYC的表达通过对OpdcyclinD 3基因的负效应与雄蕊的背腹败育有关。
结论
这些结果表明，ECE-CYC 2进化枝TCP基因不仅在背雄蕊抑制中功能保守，而且还参与了腹雄蕊的抑制，这是被子植物中，特别是在Lamiales s.l.中进化的背雄蕊和腹雄蕊都败育的两侧对称性建立的遗传机制。
Background
ECE-CYC2 clade genes known in patterning floral dorsoventral asymmetry (zygomorphy) in Antirrhinum majus are conserved in the dorsal identity function including arresting the dorsal stamen. However, it remains uncertain whether the same mechanism underlies abortion of the ventral stamens, an important morphological trait related to evolution and diversification of zygomorphy in Lamiales sensu lato, a major clade of predominantly zygomorphically flowered angiosperms. Opithandra (Gesneriaceae) is of particular interests in addressing this question as it is in the base of Lamiales s.l., an early representative of this type zygomorphy.
Results
We investigated the expression patterns of four ECE-CYC2 clade genes and two putative target cyclinD3 genes in Opithandra using RNA in situ hybridization and RT-PCR. OpdCYC gene expressions were correlated with abortion of both dorsal and ventral stamens in Opithandra, strengthened by the negatively correlated expression of their putative target OpdcyclinD3 genes. The complement of OpdcyclinD3 to OpdCYC expressions further indicated that OpdCYC expressions were related to the dorsal and ventral stamen abortion through negative effects on OpdcyclinD3 genes.
Conclusion
These results suggest that ECE-CYC2 clade TCP genes are not only functionally conserved in the dorsal stamen repression, but also involved in arresting ventral stamens, a genetic mechanism underlying the establishment of zygomorphy with abortion of both the dorsal and ventral stamens evolved in angiosperms, especially within Lamiales s.l.
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影响因子：
64.5
作者：
DOWDY, SF;HINDS, PW;WEINBERG, RA
通讯作者：
WEINBERG, RA
苦苣苔科 CYC 样花对称基因的表达分化与其蛋白序列差异相关
影响因子：
2.4
作者：
Gao, Qiu;Tao, Ju-Hong;Li, Zhen-Yu
通讯作者：
Li, Zhen-Yu
DOI：
10.1073/pnas.0801359105
发表时间：
2008-07-01
影响因子：
11.1
作者：
Broholm, Suvi K.;Tahtiharju, Sari;Elomaa, Paula
通讯作者：
Elomaa, Paula
影响因子：
64.8
作者：
Doebley, J;Stec, A;Hubbard, L
通讯作者：
Hubbard, L
DOI：
10.1073/pnas.0602827103
发表时间：
2006-06-13
影响因子：
11.1
作者：
Howarth, Dianella G.;Donoghue, Michael J.
通讯作者：
Donoghue, Michael J.