Expression Profiles of Vpx/Vpr Proteins Are Co-related with the Primate Lentiviral Lineage.
Expression Profiles of Vpx/Vpr Proteins Are Co-related with the Primate Lentiviral Lineage.
复制标题
DOI：
10.3389/fmicb.2016.01211
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Nomaguchi M
中科院分区：
文献类型：
--
作者：
Sakai Y;Miyake A;Doi N;Sasada H;Miyazaki Y;Adachi A;Nomaguchi M
人类免疫缺陷病毒2型（HIV-2）和一些猿猴免疫缺陷病毒（SIV）谱系的病毒携带一种称为Vpx的独特辅助蛋白。Vpx对于病毒在巨噬细胞和T淋巴细胞等天然靶细胞中的复制至关重要。我们已经在HIV-2和SIVmac两株病毒株中发现位于Vpx c端区域的聚脯氨酸基序（PPM）是Vpx有效表达所必需的，并且这两个克隆的Vpx表达水平有显著差异。值得注意的是，PPM序列是保守的，并且仅限于来自某些HIV-2/ siv谱系的Vpx和Vpr蛋白。本研究对不同灵长类慢病毒谱系的Vpx/Vpr蛋白进行了实验和系统发育分析，获得了Vpx/Vpr蛋白在细胞中的总体表达图谱。虽然转染细胞中Vpx/Vpr的表达水平和ppm依赖性因病毒株而异，但基于Vpx/Vpr氨基酸序列，发现每个病毒组都表现出特征表达谱。此外，Gag和Vpx/Vpr蛋白的系统发育树分析结果基本相同。综上所述，我们的研究表明，在病毒进化过程中，每种灵长类慢病毒谱系都可能发展出一种独特的Vpx/Vpr蛋白表达模式，以适应其敌对的细胞和物种环境。
Viruses of human immunodeficiency virus type 2 (HIV-2) and some simian immunodeficiency virus (SIV) lineages carry a unique accessory protein called Vpx. Vpx is essential or critical for viral replication in natural target cells such as macrophages and T lymphocytes. We have previously shown that a poly-proline motif (PPM) located at the C-terminal region of Vpx is required for its efficient expression in two strains of HIV-2 and SIVmac, and that the Vpx expression levels of the two clones are significantly different. Notably, the PPM sequence is conserved and confined to Vpx and Vpr proteins derived from certain lineages of HIV-2/SIVs. In this study, Vpx/Vpr proteins from diverse primate lentiviral lineages were experimentally and phylogenetically analyzed to obtain the general expression picture in cells. While both the level and PPM-dependency of Vpx/Vpr expression in transfected cells varied among viral strains, each viral group, based on Vpx/Vpr amino acid sequences, was found to exhibit a characteristic expression profile. Moreover, phylogenetic tree analyses on Gag and Vpx/Vpr proteins gave essentially the same results. Taken together, our study described here suggests that each primate lentiviral lineage may have developed a unique expression pattern of Vpx/Vpr proteins for adaptation to its hostile cellular and species environments in the process of viral evolution.
影响因子：
5.2
作者：
Miyake A;Miyazaki Y;Fujita M;Nomaguchi M;Adachi A
通讯作者：
Adachi A