In vivo phosphorescence imaging of pO2 using planar oxygen sensors
In vivo phosphorescence imaging of pO2 using planar oxygen sensors
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DOI：
10.1080/10739680591003314
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发表时间：
2005-09-01
期刊：
影响因子：
2.4
通讯作者：
Abels, C
中科院分区：
文献类型：
--
作者：
Babilas, P;Liebsch, G;Abels, C
目的：利用金属卟啉配合物的氧依赖猝灭发光成像技术对肿瘤和正常组织中Po(2)的分布进行成像。方法：用固定在聚苯乙烯基质中的铂(II)-八乙基卟啉作为透明平面传感器。结果：在较低的Po(2)值(0 mm Hg时为+/-0.2 mm Hg；160 mm HgPo(2)时为+/-1.5 mm Hg)范围内具有较高的灵敏度。由于通过寿命编码的内在参照，与体外校准相比，在清醒仓鼠的无色素性黑色素瘤(A-MEL-3)背部皮皱室模型中，体内的传感器响应没有修改。在正常组织(25.8+/-5.1 mm Hg)和肿瘤组织(9.2+/-5.1 mm Hg)上的Po(2)测量结果与该模型使用表面多丝电极获得的结果非常一致。结论：使用所提出的方法可以以大约100ms的高时间分辨率和至少25µm的空间分辨率来绘制表面Po(2)分布。此外，透明传感器允许同时显示潜在的微血管。
Objective: Oxygen-dependent quenching of luminescence of metal porphyrin complexes has been used to image the pO(2) distribution over tumor and normal tissue.Methods: An experimental setup is described using a platinum(II)-octaethyl-porphyrin immobilized in a polystyrene matrix as transparent planar sensor.Results: Sensitivity over a broad range is high at low pO(2) values (+/-0.2 mm Hg at 0 mm Hg; +/-1.5 mm Hg at 160 mm Hg pO(2)). Due to intrinsically referencing via lifetime encoding there was no modification of the sensor response in vivo in the dorsal skinfold chamber model with amelanotic melanoma (A-MEL-3) in awake hamsters when compared to the in vitro calibration. pO(2) measurements over normal tissue (25.8 +/- 5.1 mm Hg) and tumor tissue (9.2 +/- 5.1 mm Hg) were in excellent agreement with previous results obtained in this model using a surface multiwire electrode.Conclusions: Using the presented method the surface pO(2) distribution can be mapped with a high temporal resolution of approximately 100 ms and a spatial resolution of at least 25 mu m. Moreover, the transparent sensor allows the simultaneous visualization of the underlying microvasculature.