Aptamer based fluorescent probe for serum HER2-ECD detection: The clinical utility in breast cancer
Aptamer based fluorescent probe for serum HER2-ECD detection: The clinical utility in breast cancer
复制标题
基于适体的荧光探针用于血清 HER2-ECD 检测：在乳腺癌中的临床应用
DOI：
10.1016/j.cclet.2018.03.025
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发表时间：
2018-05-01
影响因子：
9.1
通讯作者：
Xu, Zhaochao
中科院分区：
文献类型：
--
作者：
Wang, Meiling;Yue, Dongfang;Xu, Zhaochao
跨膜蛋白HER2在大约30%的乳腺癌患者中过度表达。HER2阳性乳腺癌倾向于更具侵袭性，导致女性死亡率增加。目前，HER2状态的实时监测对HER2阳性患者的临床诊断和治疗具有重要意义。虽然IHC和FISH是评估组织HER2状态的标准方法，但这两种方法都需要高质量的组织标本，不适合实时监测HER2的状态。由于HER2受体的胞外区(ECD)可以进入循环，因此HER2ECD的血清检测已发展成为检测HER2过表达的另一种方法。血清检测将能够监测HER2状态的动态变化。本文利用Cy5标记的HB5适配子与HER2 ECD的特异结合能力，对血清HER2 ECD进行检测。这种基于适体的荧光探针易于合成和修饰，并且与抗HER2抗体一样敏感。我们认为，Cy5-HB5在血清HER2检测中具有潜在的应用潜力，可用于乳腺癌的复发和转移等临床应用。(三)2018中国化学学会、中国医学科学院药物所。爱思唯尔出版，版权所有。
The transmembrane protein HER2 is overexpressed in approximately 30% of breast cancer patients. HER2-positive breast cancers tend to spread more aggressively, which result in increased mortality in women. Nowadays, the real-time monitoring of HER2 status is important in clinical diagnosis and treatment for HER2-positive patients. Although IHC and FISH assay are standard methods to evaluate the tissue HER2 status, both approaches which required high quality tissue samples and are not suitable for monitoring the status of HER2 in real time. Since extracellular domain (ECD) of the HER2 receptor can be shed into the circulation, the serum test of HER2 ECD has been developed as an additional approach to probe HER2 overexpression. The serum test will be able to monitor the dynamic changes of HER2 status. In this paper, we detected serum HER2 ECD using Cy5-labeled HB5 aptamer as a result of its specific binding ability to HER2 ECD. This aptamer-based fluorescent probe is easily synthesized and modified and as sensitive as anti-HER2 antibodies. We believe that Cy5-HB5 may have application potentials in serum HER2 test for clinical utility of breast cancer, such as recurrence and metastases. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.