A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays
A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays
复制标题
通过配置变构转录因子识别和放大发光邻近同质测定来开发新型生物传感器的平台。
DOI：
10.1039/c6cc07244e
复制
发表时间：
2017-01-04
影响因子：
4.9
通讯作者：
Yang, Keqian
中科院分区：
文献类型：
--
作者：
Li, Shanshan;Zhou, Li;Yang, Keqian
细菌中的变构转录因子（aTF）可以检测到多种化学物质。在此，我们报告了一个生物传感平台，通过使用分离的aTF作为识别元件在体外。此外，提供了一种提高基于aTF的生物传感器的灵敏度的一般策略。作为概念验证，我们通过分别使用aTF HucR和OtrR作为识别元件获得了迄今为止最灵敏的尿酸和土霉素生物传感器。由于大量的aTF存在于细菌中，我们的工作开辟了一条新的路线，以开发敏感的aTF为基础的生物传感器。
A wide range of chemicals can be sensed by allosteric transcription factors (aTFs) in bacteria. Herein, we report a biosensing platform by using isolated aTFs as recognition elements in vitro. Moreover, a general strategy to increase the sensitivity of the aTF-based biosensors is provided. As a proof-of-concept, we obtained by far the most sensitive uric acid and oxytetracycline biosensors by using aTF HucR and OtrR as recognition elements, respectively. As a large number of aTFs are present in bacteria, our work opens a novel route to develop sensitive aTF-based biosensors.