FORMATION AND PROPERTIES OF A SYNTHETIC BONE COMPOSITE - HYDROXYAPATITE-COLLAGEN
FORMATION AND PROPERTIES OF A SYNTHETIC BONE COMPOSITE - HYDROXYAPATITE-COLLAGEN
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DOI：
10.1002/jbm.820290704
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发表时间：
1995-07-01
期刊：
影响因子：
--
通讯作者：
BROWN, PW
中科院分区：
文献类型：
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作者：
TENHUISEN, KS;MARTIN, RI;BROWN, PW
由微晶缺钙羟基磷灰石 (HAp) 和胶原蛋白组成的复合材料是在胶原蛋白基质存在的情况下，通过磷酸钙前体之间的酸碱反应在 38 摄氏度下形成的。研究了 HAp:胶原重量比为 4.5:1、11:1 和 22:1 的复合材料以及纯矿物质的形成。等温量热法和 X 射线衍射表明反应在 5 小时内完成，形成硬化的整体材料。 HAp 形成的速率随着胶原蛋白比例的增加而增加。电子显微镜表明胶原蛋白的加速作用与为HAp结晶提供成核位点有关。溶液化学分析还表明胶原蛋白影响钙和磷酸盐浓度以及 pH 值。虽然胶原蛋白被证明会影响 HAp 形成的动力学，但如 X 射线衍射和溶液化学所示，限速步骤是酸性磷酸钙前体 CaHPO4 的溶解。初步机械数据表明，杨氏模量、屈服强度和断裂功处于所报告的骨值范围的下限。在这些复合材料中观察到的孔隙率表明它们可能具有骨诱导性，而它们的成分应允许它们最终吸收。因此，微观结构、动力学和机械数据表明这些复合材料适合作为体内形成的骨替代物。 (C) 1995 约翰威利父子公司
Composites composed of microcrystalline calcium-deficient hydroxyapatite (HAp) and collagen were formed at 38 degrees C via an acid-base reaction between calcium phosphate precursors in the presence of a collagen matrix. Formation of composites having HAp:collagen weight ratios of 4.5:1, 11:1, and 22:1, along with that of pure mineral were investigated. Isothermal calorimetry and X-ray diffraction indicated complete reaction within 5 h resulting in hardened monoliths. The rate of HAp formation increased with an increase in the proportion of collagen present. Electron microscopy indicated that the acceleratory effect of collagen was associated with the provision of nucleation sites for HAp crystallization. Analysis of the solution chemistry also showed that collagen affected the calcium and phosphate concentrations and the pH. While collagen was shown to effect the kinetics of HAp formation, the rate limiting step, as shown by X-ray diffraction and solution chemistry, was the dissolution of the acidic calcium phosphate precursor, CaHPO4. Preliminary mechanical data indicated that the Young's modulus, yield strength, and work to fracture were at the lower end of the range of those values reported for bone. The porosities observed in these composites suggest that they might be osteoinductive while their compositions should allow their eventual resorption. Thus, microstructure, kinetics, and mechanical data suggest that these composite-night be suitable as bone substitutes which form in vivo. (C) 1995 John Wiley and Sons, Inc.