Dose and airflow dependence of benzyl alcohol disposition on skin.
Dose and airflow dependence of benzyl alcohol disposition on skin.
复制标题
苯甲醇在皮肤上的分布的剂量和气流依赖性。
DOI：
10.1002/jps.20513
复制
发表时间：
2006
影响因子：
3.8
通讯作者：
Kasting,GeraldB
中科院分区：
文献类型：
--
作者：
Miller,MatthewA;Bhatt,Varsha;Kasting,GeraldB
在放置在通风柜中的未闭塞的Franz细胞中，测量了苯甲醇（BA）通过裂厚尸体皮肤的渗透。BA溶解在小体积乙醇中，加入14c放射性标签，以0.9至10600 μ g/cm2的9种剂量施用于皮肤。24 h后放射性穿透率随剂量逐渐增大，最低剂量为19.8±2.9%，最高剂量为29.2±3.0%。24 h时，组织中残留的放射性不到4%；其余的被认为是蒸发了。根据有限剂量扩散/蒸发模型对这些数据和先前研究的数据进行了分析。分析表明，BA吸收随剂量的增加与角质层BA扩散系数随其浓度从示踪剂水平增加到饱和而增加三倍相一致。可变扩散率模型能够描述两项研究的综合观测结果，其均方根误差在剂量的4.2%以内。发现了一种独立于实验估算扩散模型参数的方法，该方法在低剂量和中剂量下与实验得出的值很好地吻合。©2005 Wiley‐Liss, Inc. and American Pharmacists Association . J pharmaceutical science, 95:281-291, 2006
The penetration of benzyl alcohol (BA) through split‐thickness cadaver skin was measured in nonoccluded Franz cells placed in a fume hood. BA, dissolved in a small volume of ethanol and spiked with14C radiolabel, was applied to skin at nine doses ranging from 0.9 to 10600 µg/cm2. The percentage of radioactivity penetrated after 24 h increased gradually with dose, ranging from 19.8 ± 2.9% at the lowest dose to 29.2 ± 3.0% at the highest. Less than 4% of the radioactivity was retained in the tissue at 24 h; the remainder was considered to be evaporated. These data and those from a previous study were analyzed in terms of a finite dose diffusion/evaporation model. The analysis showed that the increase in BA absorption with dose was consistent with a threefold increase in BA diffusivity in the stratum corneum, as its concentration increased from tracer levels to saturation. The variable diffusivity model was able to describe the combined observations from the two studies to within an rms error of 4.2% of dose. A method of estimating the diffusion model parameters independently of the experiment was found to yield good agreement with the experimentally‐derived values at low and moderate doses. © 2005 Wiley‐Liss, Inc. and the American Pharmacists Association J Pharm Sci 95:281–291, 2006