Notch signaling regulates the expression of glycolysis-related genes in a context-dependent manner during embryonic development
Notch signaling regulates the expression of glycolysis-related genes in a context-dependent manner during embryonic development
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DOI：
10.1016/j.bbrc.2018.06.079
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发表时间：
2018-09-05
影响因子：
3.1
通讯作者：
Itoh, Motoyuki
中科院分区：
文献类型：
--
作者：
Kuwabara, Shuhei;Yamaki, Misato;Itoh, Motoyuki
糖酵解是产生能量的经典途径，已知与神经发育有关。Notch信号还参与各种组织的神经发育和糖酵解调节。然而，Notch信号在神经发育过程中糖酵解相关基因调控中的作用尚不清楚。在这里，我们使用斑马鱼mib1（ta52b）突变体分析了糖酵解中葡萄糖转运体（GLUT）和限速酶的mRNA表达模式和水平，这些突变体在胚胎早期和幼虫阶段缺乏Notch信号。我们的研究结果表明，在神经组织中，Notch信号在幼虫期正调节glutla和glut3a的表达，负调节hk2的表达，但在胚胎早期可能不调节它们。因此，这些结果表明Notch信号在不同发育阶段的神经组织中以上下文依赖的方式调节糖酵解相关基因的表达。(C) 2018爱思唯尔公司版权所有。
Glycolysis, the classic pathway for producing energy, has been known to be involved in neural development. Notch signaling also contributes to neural development and regulation of glycolysis in various tissues. However, the role of Notch signaling in glycolysis-related gene regulation during neural development is poorly understood. Here, we analyzed mRNA expression patterns and levels of glucose transporters (GLUT) as well as rate-limiting enzymes in glycolysis using zebrafish mib1(ta52b) mutants, in which Notch signaling was deficient at the early embryonic and larval stages. Our results indicated that in neural tissues, Notch signaling positively regulates glutla and glut3a expression and negatively regulates hk2 expression at the larval stage but may not regulate them during early embryonic stages. Therefore, these results suggest that Notch signaling regulates glycolysis-related gene expression in a context-dependent manner in neural tissues at different developmental stages. (C) 2018 Elsevier Inc. All rights reserved.