MassTag polymerase-chain-reaction detection of respiratory pathogens, including a new rhinovirus genotype, that caused influenza-like illness in New York State during 2004-2005
MassTag polymerase-chain-reaction detection of respiratory pathogens, including a new rhinovirus genotype, that caused influenza-like illness in New York State during 2004-2005
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DOI：
10.1086/508551
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发表时间：
2006-11-15
影响因子：
6.4
通讯作者：
Lipkin, W. Ian
中科院分区：
文献类型：
--
作者：
Lamson, Daryl;Renwick, Neil;Lipkin, W. Ian
2004年冬季，在纽约州，流感样疾病的发病率很高，通过分子方法检测流感病毒和通过病毒培养检测其他呼吸道病毒均为阴性。由于担心可能涉及一种新的病原体，我们采用了一种新的多重诊断工具。MassTag聚合酶链反应解决了79个先前阴性样本中的26个，揭示了大部分样本中存在鼻病毒，其中一半属于以前未表征的遗传分支。在某些情况下，对检测到的病毒和/或细菌（共）感染的了解可能会改变临床管理。
In New York State during winter 2004, there was a high incidence of influenza-like illness that tested negative both for influenza virus, by molecular methods, and for other respiratory viruses, by virus culture. Concern that a novel pathogen might be implicated led us to implement a new multiplex diagnostic tool. MassTag polymerase chain reaction resolved 26 of 79 previously negative samples, revealing the presence of rhinoviruses in a large proportion of samples, half of which belonged to a previously uncharacterized genetic clade. In some instances, knowledge of the detected viral and/or bacterial (co) infection could have altered clinical management.