Dynamic Extracellular Imaging of Biochemical Cell Activity Using InGaN/GaN Nanowire Arrays as Nanophotonic Probes
Dynamic Extracellular Imaging of Biochemical Cell Activity Using InGaN/GaN Nanowire Arrays as Nanophotonic Probes
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DOI：
10.1002/adfm.201802503
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发表时间：
2018-09-26
影响因子：
19
通讯作者：
Eickhoff, Martin
中科院分区：
文献类型：
--
作者：
Hoelzel, Sara;Zyuzin, Mikhail V.;Eickhoff, Martin
InGaN/GaN纳米线异质结构阵列作为光子探针的生物化学和细胞过程中的入射光荧光显微镜的动态成像的应用程序被证明。InGaN/GaN纳米线的光致发光强度敏感地依赖于周围溶液的pH值，使它们成为伴随局部pH变化的生物化学过程的光学检测的合适探针。生长在导电衬底上的纳米线阵列可以在明确定义的电化学工作点中操作，具有高灵敏度和稳定性。可实现的pH值和偏差分辨率以及信噪比作为工作点的函数和不同的积分时间进行评估。1 mV的偏压分辨率和0.03的pH值的分辨率，实现在低于25 ms的时间分辨率。应用程序的动态成像的活动，从Wistar大鼠的离体肠隐窝。在这里，在隐窝附近的pH值的变化是定量的，并归因于钠质子交换剂（NHE）的活性。阿米洛利和NH 4Cl对其活性的影响的成像显示为空间分辨率为
The application of InGaN/GaN nanowire heterostructure arrays as photonic probes for dynamic imaging of biochemical and cellular processes in an incident light fluorescence microscope is demonstrated. The photoluminescence intensity of InGaN/GaN nanowires sensitively depends on the pH value of the surrounding solution, making them suitable probes for the optical detection of biochemical processes accompanied by local pH variations. Grown on a conductive substrate, the nanowire arrays can be operated in a well-defined electrochemical working point with high sensitivity and stability. The achievable pH and bias resolution as well as signal-to-noise ratio are assessed as a function of the working point and for different integration times. A bias resolution of 1 mV and a pH resolution of 0.03 are achieved at a time resolution below 25 ms. The application for dynamic imaging of the activity of isolated intestinal crypts from Wistar rats is demonstrated. Here, the pH change in the vicinity of the crypt is quantified and attributed to the activity of the sodium-proton exchanger (NHE). Imaging of the effect of amiloride and NH4Cl on its activity is demonstrated with a spatial resolution of