Mixed Gastric- and Intestinal-type Metaplasia Is Formed by Cells with Dual Intestinal and Gastric Differentiation
Mixed Gastric- and Intestinal-type Metaplasia Is Formed by Cells with Dual Intestinal and Gastric Differentiation
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DOI：
10.1177/002215540505300109
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发表时间：
2005-01
影响因子：
3.2
通讯作者：
T. Niwa;Y. Ikehara;H. Nakanishi;Harunari Tanaka;K. Inada;T. Tsukamoto;M. Ichinose;M. Tatematsu
中科院分区：
文献类型：
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作者：
T. Niwa;Y. Ikehara;H. Nakanishi;Harunari Tanaka;K. Inada;T. Tsukamoto;M. Ichinose;M. Tatematsu
我们建议根据残留的胃表型细胞，将肠化生（IM）分为胃肠混合型（GI）和单肠型(I)两类。gi混合型IM可以通过在单个腺体中存在胃或肠表型的两种细胞来识别。本研究旨在阐明gi混合型IM腺体中的细胞是否可以同时呈现胃和肠表型。采用AlexaFluor 488或568标记特异性单克隆抗体直接检测5例胃癌20例标本中MUC5AC、MUC2、CD10和绒毛蛋白的表达，采用荧光显微镜和激光共聚焦扫描显微镜观察。gi混合IM腺体包括表达MUC5AC和MUC2、MUC5AC和绒毛蛋白、MUC5AC和CD10的群体。MUC2和绒毛蛋白的表达随MUC5AC表达的降低而相互增加，而CD10的表达仅限于MUC5AC仅残留表达或不表达的细胞。这些结果表明，具有胃和肠两种表型的异质细胞群会发展成单一的肠道表型，这反映在肠化生从gi混合型到i型im型腺体的进展中。
We have proposed to divide intestinal metaplasia (IM) into two categories, i.e., a mixed gastric and intestinal (GI) type, and a solely intestinal (I) type, based on the residual gastric phenotype cells. The GI-mixed-type IM can be identified by the presence of both cells with either gastric or intestinal phenotypes in a single gland. This study is conducted to elucidate whether cells in the GI-mixed-type IM glands can simultaneously present both gastric and intestinal phenotypes. MUC5AC, MUC2, CD10 and villin expressions were investigated in 20 samples from five gastric cancer cases, directly using either AlexaFluor 488- or 568-labeled specific monoclonal antibodies and observed by fluorescent microscopy and confocal laser-scanning microscopy. GI-mixed IM glands comprise a population expressing MUC5AC and MUC2, MUC5AC and villin, and MUC5AC and CD10. MUC2 and villin expressions were reciprocally increased with decreasing MUC5AC expression, while CD10 expression was limited to cells with only a residual MUC5AC expression or no expression. These results suggest that a heterogeneous cell population with both gastric and intestinal phenotypes would develop into a single intestinal phenotype, as reflected in the progression of intestinal metaplasia from GI-mixed-type- to I-type IM-type glands.