Chronic Ethanol Exposure Effects on Vitamin D Levels Among Subjects with Alcohol Use Disorder.
Chronic Ethanol Exposure Effects on Vitamin D Levels Among Subjects with Alcohol Use Disorder.
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DOI：
10.4137/ehi.s40335
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发表时间：
2016
影响因子：
2.7
通讯作者：
McCaskill M
中科院分区：
文献类型：
--
作者：
Ogunsakin O;Hottor T;Mehta A;Lichtveld M;McCaskill M
维生素D此前已被认为在人体免疫系统和功能中发挥重要作用。在肺系统中，维生素D调节抗菌肽的功能，尤其是中草药/LL-37。人盲肠毒素/LL-37是一种具有保护免疫功能的杀菌、抑菌、抗病毒内源性多肽。长期接触过量酒精有可能降低维生素D(非活性维生素D[25(OH)D3]和活性维生素D[1，25(OH)2D3])水平，并导致放线菌素/LL-37的下调。酒精介导的LL-37的减少可能是酒精使用障碍(AUD)患者更频繁和更严重的呼吸道感染发生率增加的部分原因。本研究的目的是探讨酒精对维生素D代谢的影响机制。此外，研究目的是利用AUD受试者和健康对照组的支气管肺泡灌洗液样本，建立慢性酒精暴露、肺维生素D水平和放线菌素/IL-37之间的联系。实验结果显示，非活性维生素D(25(OH)D3)、活性维生素D(1，25(OH)2D3)、放线菌素/LL-37和细胞色素P27B1蛋白水平显著低于健康对照组(P<；0.05)。然而，所有被检测的样本中，细胞色素P450-2E_1均升高。长期接触酒精有可能降低肺中维生素D的水平，并导致人体肺部系统中抗菌肽LL-37的随后下调。
Vitamin D has been previously recognized to play important roles in human immune system and function. In the pulmonary system, vitamin D regulates the function of antimicrobial peptides, especially cathelicidin/LL-37. Human cathelicidin/LL-37 is a bactericidal, bacteriostatic, and antiviral endogenous peptide with protective immune functions. Chronic exposure to excessive alcohol has the potential to reduce levels of vitamin D (inactive vitamin D [25(OH)D3] and active vitamin D [1, 25(OH)2D3]) and leads to downregulation of cathelicidin/LL-37. Alcohol-mediated reduction of LL-37 may be partly responsible for increased incidence of more frequent and severe respiratory infections among subjects with alcohol use disorder (AUD). The objective of this study was to investigate the mechanisms by which alcohol exerts its influence on vitamin D metabolism. In addition, the aim was to establish associations between chronic alcohol exposures, levels of pulmonary vitamin D, and cathelicidin/LL-37 using broncho-alveolar lavage fluid samples of subjects with AUD and healthy controls. Findings from the experiment showed that levels of inactive vitamin D (25(OH)D3), active vitamin D (1, 25(OH)2D3), cathelicidin/LL-37, and CYP27B1 proteins were significantly reduced (P < 0.05) when compared with the matched healthy control group. However, CYP2E1 was elevated in all the samples examined. Chronic exposure to alcohol has the potential to reduce the levels of pulmonary vitamin D and results in subsequent downregulation of the antimicrobial peptide, LL-37, in the human pulmonary system.