Frequency and stability of chromosomal integration of adenovirus vectors
Frequency and stability of chromosomal integration of adenovirus vectors
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DOI：
10.1128/jvi.73.7.6141-6146.1999
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发表时间：
1999-07-01
影响因子：
5.4
通讯作者：
Mitani, K
中科院分区：
文献类型：
--
作者：
Harui, A;Suzuki, S;Mitani, K
腺病毒载体的局限性之一是缺乏必要的机制来整合到宿主染色体中，导致在分裂细胞中短期的基因表达。我们分析了整合腺病毒载体的整合频率和基因表达的持久性。EL-替换腺病毒载体和辅助子依赖的腺病毒载体获得了相似的整合效率，每细胞10(-3)-10(-5)，辅助子依赖的载体显示出略高的整合效率。在不稳定的细胞库中，整合载体的基因表达持续了至少50个细胞分裂，没有选择。然而，一些稳定的细胞克隆表现出基因表达的变化，并伴随着整合载体DNA的结构变化。
One of the limitations of adenovirus vectors is the lack of machinery necessary for their integration into host chromosomes, resulting in short-term gene expression in dividing cells. We analyzed frequencies of integration and persistence of gene expression from integrated adenovirus vectors. Both El-substituted and helper-dependent adenovirus vectors achieved similar integration efficiencies of similar to 10(-3) to 10(-5) per cell, with the helper-dependent vector showing slightly higher efficiencies. Un stable cell pools, gene expression of the integrated vector persisted for at least 50 cell divisions without selection. However, some stable cell clones showed changes in gene expression, which were accompanied by structural changes in the integrated vector DNA.