A simplified method for isolating highly purified neurons, oligodendrocytes, astrocytes, and microglia from the same human fetal brain tissue
A simplified method for isolating highly purified neurons, oligodendrocytes, astrocytes, and microglia from the same human fetal brain tissue
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DOI：
10.1007/s11064-007-9340-y
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发表时间：
2007-12-01
影响因子：
4.4
通讯作者：
Pahan, Kalipada
中科院分区：
文献类型：
--
作者：
Jana, Malabendu;Jana, Arundhati;Pahan, Kalipada
阐明导致神经元和少突胶质细胞凋亡以及小胶质细胞和星形胶质细胞在不同神经退行性和神经炎性疾病中活化的潜在致病机制仍然是神经科学中的挑战。为了克服挑战并找到治疗方法，重要的是研究大脑中每种细胞类型的生存和死亡过程。在这里，我们提出了一个协议，分离高度纯化的小胶质细胞，星形胶质细胞，少突胶质细胞和神经元，所有四种主要的细胞类型的中枢神经系统，从同一人胎脑组织。如在体内发现的，这些原代神经元和少突胶质细胞在响应神经退行性挑战时经历凋亡和细胞死亡。另一方面，星形胶质细胞和小胶质细胞，在神经退行性大脑中不死亡的细胞，在炎症刺激后被激活。高度纯化的人脑细胞的可用性将增加开发不同神经退行性疾病疗法的可能性。
Elucidation of the underlying pathogenic mechanisms leading to apoptosis of neurons and oligodendrocytes and activation of microglia and astrocytes in different neurodegenerative and neuroinflammatory disorders remains a challenge in neuroscience. In order to overcome the challenge and find out therapeutic remedies, it is important to study live and death processes in each and every cell type of the brain. Here we present a protocol of isolating highly purified microglia, astrocytes, oligodendrocytes, and neurons, all four major cell types of the CNS, from the same human fetal brain tissue. As found in vivo, these primary neurons and oligodendroglia underwent apoptosis and cell death in response to neurodegenerative challenges. On the other hand, astroglia, and microglia, cells that do not die in neurodegenerative brains, became activated after inflammatory challenge. The availability of highly purified human brain cells will increase the possibility of developing therapies for different neurodegenerative disorders.