Retroregulation: control of integrase expression by the b2 region of bacteriophages lambda and 434.
Retroregulation: control of integrase expression by the b2 region of bacteriophages lambda and 434.
复制标题
逆向调节：通过噬菌体 lambda 和 434 的 b2 区域控制整合酶表达。
DOI：
10.1016/0042-6822(83)90293-3
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发表时间：
1983
期刊：
影响因子：
3.7
通讯作者：
Campbell,A
中科院分区：
文献类型：
--
作者：
Mascarenhas,D;Trueheart,J;Benedik,M;Campbell,A
利用体内和体外技术构建的遗传融合体，研究了b2dna的250碱基对片段在三种不同启动子PI， PLand， PTRP的转录本表达中的调控作用。已经确定(a)产生功能性整合蛋白的pif和ptrpr转录本被b2片段有效终止，(b)在同一测试系统中，不产生功能性整合酶的pl转录本不被b2片段终止，(c)这种b2（sib）效应不依赖于位于噬菌体基因组之间的基因，(d)这种效应也在噬菌体434的b2dna上观察到。它类似于λ在-197和- atti之间的表达式，但与它在-197基底左边的表达式大相径庭。
Genetic fusions constructed by a combination ofin vivoandin vitrotechniques have been used to investigate thecis-regulatory role of a 250-base-pair segment ofb2DNA in the expression ofinttranscripts originating from three different promoters PI, PLand PTRP. It has been established that (a) the PIand PTRPtranscripts, which produce functionalintprotein, are efficiently terminated by theb2segment, (b) in the same test system, the PLtranscript, which does not result in functional integrase, is not terminated by theb2segment, (c) thisb2(sib) effect does not depend on genes lying betweenintandNon the phage genome, (d) thesibeffect is also observed with theb2DNA of phage 434, which resembles that of λ between -197 andattbut diverges radically from it to the left of base -197.