Serum Sclerostin Increases in Healthy Adult Men during Bed Rest
Serum Sclerostin Increases in Healthy Adult Men during Bed Rest
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DOI：
10.1210/jc.2012-1579
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发表时间：
2012-09-01
影响因子：
5.8
通讯作者：
Smith, S. M.
中科院分区：
文献类型：
--
作者：
Spatz, J. M.;Fields, E. E.;Smith, S. M.
内容：动物模型和人体研究表明，骨细胞调节骨骼的机械卸载的反应，部分通过增加sclerostin。然而，很少有研究报道了暴露于降低机械负荷的人类血清sclerostin的变化。目的：我们确定了接受控制性卧床休息的健康成年男性血清sclerostin和骨转换标志物的变化。设计、设置和参与者：七个健康的成年男子（31 +/- 3岁）在德克萨斯大学医学转化科学研究所分支接受了90天的6次头低位倾斜卧床休息-结果：在卧床休息前[基线（BL）]和卧床休息第28天（BR-28）、第60天（BR-60）和第90天（BR-90）评价血清硬化素、PTH、维生素D、骨吸收和骨形成标志物、尿钙和磷排泄以及24小时合并尿骨吸收标志物。在BL、BR-60和研究结束后5天（BR+5）测量骨密度。数据报告为平均值+/- SD.Results：与以前的报告一致，骨矿物质密度下降显着（每月1-2%），在承重骨骼网站。血清硬化蛋白在BR-28（+29 +/-20%; P = 0.003）和BR-60（+42 +/-31%; P < 0.001）时升高至BL以上，在BR-90（+22 +/-21%; P = 0.07）时增加较少。血清PTH水平在BR-28（-17 +/- 16%; P = 0.02）和BR-60（-24 +/- 14%; P = 0.03）时降低，在BR-90（-21 +/- 21%; P = 0.14）时仍低于BL，但未达到统计学显著性。结论：卧床90 d后，健康男性血清硬化素水平升高，60 d时达到高峰，而血清甲状旁腺素水平下降，尿钙和骨吸收指标升高。（临床内分泌代谢杂志97：E1736-E1740，2012）
Context: Animal models and human studies suggest that osteocytes regulate the skeleton's response to mechanical unloading in part by an increase in sclerostin. However, few studies have reported changes in serum sclerostin in humans exposed to reduced mechanical loading.Objective: We determined changes in serum sclerostin and bone turnover markers in healthy adult men undergoing controlled bed rest.Design, Setting, and Participants: Seven healthy adult men (31 +/- 3 yr old) underwent 90 d of 6 head down tilt bed rest at the University of Texas Medical Branch Institute for Translational Sciences-Clinical Research Center.Outcomes: Serum sclerostin, PTH, vitamin D, bone resorption and formation markers, urinary calcium and phosphorus excretion, and 24-h pooled urinary markers of bone resorption were evaluated before bed rest [baseline (BL)] and at bed rest d 28 (BR-28), d 60 (BR-60), and d 90 (BR-90). Bone mineral density was measured at BL, BR-60, and 5 d after the end of the study (BR+5). Data are reported as mean +/- SD.Results: Consistent with prior reports, bone mineral density declined significantly (1-2% per month) at weight-bearing skeletal sites. Serum sclerostin was elevated above BL at BR-28 (+29 +/- 20%; P = 0.003) and BR-60 (+42 +/- 31%; P < 0.001), with a lesser increase at BR-90 (+22 +/- 21%; P = 0.07). Serum PTH levels were reduced at BR-28 (-17 +/- 16%; P = 0.02) and BR-60 (-24 +/- 14%; P = 0.03) and remained lower than BL at BR-90 (-21 +/- 21%; P = 0.14), but did not reach statistical significance. Serum bone turnover markers were unchanged; however, urinary bone resorption markers and calcium were significantly elevated at all time points after bed rest (P < 0.01).Conclusions: In healthy men subjected to controlled bed rest for 90 d, serum sclerostin increased, with a peak at 60, whereas serum PTH declined, and urinary calcium and bone resorption markers increased. (J Clin Endocrinol Metab 97: E1736-E1740, 2012)