Reciprocal transmembranous receptor-cytoskeleton interactions in concanavalin A-activated platelets.
Reciprocal transmembranous receptor-cytoskeleton interactions in concanavalin A-activated platelets.
复制标题
伴刀豆球蛋白 A 激活血小板中的相互跨膜受体-细胞骨架相互作用。
DOI：
10.1083/jcb.101.3.993
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Carroll,RC
中科院分区：
文献类型：
--
作者：
Wheeler,ME;Gerrard,JM;Carroll,RC
伴刀豆球蛋白A（ConA）已被用于活化血小板，诱导糖蛋白IIb-IIIa复合物与活化血小板的细胞骨架之间的特异性相互作用。与此一致，我们已经表明，Con A激活人血小板，启动磷酸化，分泌和细胞骨架的形成。Con A和细胞松弛素B用于证明糖蛋白复合物与血小板细胞骨架的相互作用。此外，我们已经表明，在凝血酶诱导的凝块中发现的多价纤维蛋白-纤维蛋白原血小板相互作用提供了类似的互惠性。尽管前列腺素E1完全抑制血小板活化，但Con A与其他活化剂不同，它沉淀了明显的细胞骨架核心。我们建议，从这个结果，刀豆蛋白A可能是交联的膜相关的细胞骨架复合物存在于未活化的血小板。
Concanavalin A (Con A) has been used to activate platelets, inducing a specific interaction between the glycoprotein IIb-IIIa complex and the cytoskeleton of the activated platelet. In agreement with this, we have shown that Con A activates human platelets, initiating phosphorylation, secretion, and cytoskeletal formation. Con A and cytochalasin B were used to demonstrate a reciprocal interaction of the glycoprotein complex with the platelet cytoskeleton. Additionally, we have shown that a similar reciprocity is provided by the multivalent fibrin-fibrinogen platelet interaction found in the thrombin-induced clot. Con A differs from other activators in precipitating an apparent cytoskeletal core despite a complete inhibition of platelet activation by prostaglandin E1. We suggest, from this result, that Con A may be cross-linking a membrane-associated cytoskeletal complex present in the unactivated platelet.