Comparative reactivities of diolepoxide metabolites of carcinogenic hydrocarbons with phiX174 viral DNA.
Comparative reactivities of diolepoxide metabolites of carcinogenic hydrocarbons with phiX174 viral DNA.
复制标题
致癌碳氢化合物的二醇环氧化物代谢物与 phiX174 病毒 DNA 的反应性比较。
DOI：
10.1016/0006-291x(79)91723-6
复制
发表时间：
1979
影响因子：
3.1
通讯作者：
S. B. Weiss
中科院分区：
文献类型：
--
作者：
W. Hsu;E. Lin;P. Fu;R. Harvey;S. B. Weiss
先前开发的ØX174 DNA分析系统用于测定一系列不同致癌能力的多环芳烃的二氯环氧化合物代谢物与核酸的比较反应性。将感染性ØX174病毒DNA暴露于碳氢化合物衍生物中10分钟，然后通过与大肠杆菌球质体孵育，计数琼脂板上的斑块形成，来检测处理过的DNA的感染性。湾区苯并[a]-芘、蒽和二苯并[a， h]蒽的二聚环氧化合物，被认为是母体碳氢化合物的最终活性致癌代谢物，表现出强大的病毒抑制活性。另一方面，病毒抑制活性与二聚环氧化物在海湾区域的位置或理论计算的打开环氧化物环产生的碳离子的β-离域能（ΔE deloc.）之间没有明显的相关性。讨论了这些发现对致癌理论的意义。
Abstract The ØX174 DNA assay system developed earlier is utilized to determine the comparative reactivities with nucleic acid of the diolepoxide metabolites of a series of polycyclic aromatic hydrocarbons varying in carcinogenic potency. The infectious ØX174 viral DNA is exposed to the hydrocarbon derivative for 10 min., then infectivity of the treated DNA is assayed by incubation with E. coli spheroplasts, counting plaque formation on agar plates. The bay region diolepoxides of benzo [a]-pyrene, chrysene, and dibenz [a, h] anthracene, implicated as the ultimately active carcinogenic metabolites of the parent hydrocarbons, exhibit potent viral inhibitory activity. On the other hand no correlation is evident between viral inhibitory activity and either the location of the diolepoxide function in a bay region or the theoretically calculated β-delocalization energies (ΔE deloc.) of the carbonium ion arising from opening the epoxide ring. The significance of these findings with respect to theories of carcinogenesis is discussed.