Biodistribution, pharmacokinetics and PET Imaging of [18F]FMISO, [18F] FDG and [18F]FAc in a sarcoma- and inflammation-bearing mouse model
Biodistribution, pharmacokinetics and PET Imaging of [18F]FMISO, [18F] FDG and [18F]FAc in a sarcoma- and inflammation-bearing mouse model
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DOI：
10.1016/j.nucmedbio.2008.12.011
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发表时间：
2009-04-01
影响因子：
3.1
通讯作者：
Wang, Hsin-Ell
中科院分区：
文献类型：
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作者：
Liu, Ren-Shyan;Chou, Ta-Kai;Wang, Hsin-Ell
2-脱氧-2-[(18)F]氟-D-葡萄糖([(18)F]FDG)、[(18)F]氟乙酸盐[(18)F]FAc)和[(18)F]氟米索硝唑([(18)F)FMISO)都被认为是用于肿瘤诊断的正电子发射断层扫描(PET)探针，尽管基于不同的组织摄取原理。本研究比较了这三种示踪剂在肉瘤和炎症抑制小鼠模型中的生物分布、药代动力学和成像。方法：第0天在C3H小鼠的右大腿接种2x10（5）个KHT肉瘤细胞。第11天在左大腿注射松节油（0.1 ml）以诱导炎症病变。肿瘤接种后第14天进行[(18)F]FMISO、[(18)F]FDG和[18F]FAc的生物分布、药代动力学和microPET成像。结果：松节油注射后3天，通过[(18)F]FDG/microPET和放射自显影清楚地显示炎症病变。注射后 4 小时，由 microPET 成像得出的肿瘤与肌肉和炎症病变与肌肉的比率分别为：[(18)F] FMISO 为 6.79 和 1.48，[(18)F]FDG 为 8.12 和 4.69，[(18)F]FAc 为 3.72 和 3.19。其中，[(18)F]FMISO 的肿瘤与炎症比率最高 (4.57)，而 [(18)F]FDG (1.73) 和 [(18)F]FAc (1.17) 的肿瘤与炎症比率最高，而 [(18)F]FAc 的生物利用度最高（放射性示踪剂浓度与时间曲线下的面积，116.2 hx 每粒注射剂量的百分比）结论：MicroPET图像和生物分布研究表明，注射后4小时，[(18)F]FMISO在肿瘤中的积累明显高于炎症病灶中的积累。 [(18)F]FDG 和 [(18)F]FAc 描绘了肿瘤和炎症病变。我们的结果证明了 [(18)F]FMISO/PET 在区分肿瘤和炎症病变方面的潜力。 (C) 2009 Elsevier Inc. 保留所有权利。
2-Deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG), [(18)F]fluoroacetate [(18)F]FAc) and [(18)F]fluoromisonidazole ([(18)F)FMISO) were all considered to be positron emission tomography (PET) probes for tumor diagnosis, though based on different rationale of tissue uptake. This study compared the biodistribution, pharmacokinetics and imaging of these three tracers in a sarcoma- and inflammation-beating mouse model.Methods: C3H mice were inoculated with 2x10(5) KHT sarcoma cells in the right thigh on Day 0. Turpentine oil (0.1 ml) was injected in the left thigh on Day 11 to induce inflammatory lesion. Biodistribution, pharmacokinetics and microPET imaging of [(18)F]FMISO, [(18)F]FDG and [18F]FAc were performed on Day 14 after tumor inoculation.Results: The inflammatory lesions were clearly visualized by [(18)F]FDG/microPET and autoradiography at 3 days after turpentine oil injection. The tumor-to-muscle and inflammatory lesion-to-muscle ratios derived from microPET imaging were 6.79 and 1.48 for [(18)F] FMISO, 8.12 and 4.69 for [(18)F]FDG and 3.72 and 3.19 for [(18)F]FAc at 4 h post injection, respectively. Among these, the tumor-to-inflammation ratio was the highest(4.57) for [(18)F]FMISO compared with that of [(18)F]FDG (1.73)and [(18)F]FAc (1.17), whereas [(18)F]FAc has the highest bioavailability (area under concentration of radiotracer vs. time curve, 116.2 hxpercentage of injected dose per grain of tissue).Conclusions: MicroPET images and biodistribution studies showed that the accumulation of [(18)F]FMISO in the tumor is significantly higher than that in inflammatory lesion at 4 h post injection. [(18)F]FDG and [(18)F]FAc delineated both tumor and inflammatory lesions. Our results demonstrated the potential of [(18)F]FMISO/PET in distinguishing tumor from inflammatory lesion. (C) 2009 Elsevier Inc. All rights reserved.