A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions.
A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions.
复制标题
人内皮细胞限制的外部处置的浆膜蛋白，富含细胞间连接。
DOI：
10.1084/jem.170.2.399
复制
发表时间：
1989-08-01
影响因子：
15.3
通讯作者：
Cohn, Z A
中科院分区：
文献类型：
--
作者：
Muller, W A;Ratti, C M;McDonnell, S L;Cohn, Z A
我们已经提出了一个mAb，以前未描述的135 kD外部设置的膜蛋白，是丰富的细胞间连接结构域培养的人脐静脉内皮细胞。这种蛋白质定位于细胞汇合时的并置表面，并在汇合单层的交界区稳定表达。该蛋白质在所有检查的人体组织中的所有血管的连续内皮上原位表达。此外，这种蛋白质，如通过mAb免疫细胞化学所确定的，不被任何其他细胞类型表达。这种蛋白质可能介导内皮细胞特异性功能，仅限于细胞间结构域。它也可以作为一种独特的细胞表面标记物，用于人内皮细胞的鉴定和纯化。
We have raised an mAb to a previously undescribed 135-kD externally disposed integral membrane protein that is enriched in the intercellular junctional domain of cultured human umbilical vein endothelial cells. This protein localizes at the appositional surfaces of cells as they become confluent and is stably expressed in the junctional zones of confluent monolayers. This protein is expressed in situ on continuous endothelia of all blood vessels in all human tissues examined. Moreover, this protein, as determined by mAb immunocytochemistry, is not expressed by any other cell type. This protein may mediate endothelial-specific functions restricted to the intercellular domain. It may also serve as a unique cell surface marker for the identification and purification of human endothelial cells.