Effects of eight InDel variants in FHIT on milk traits in Xinjiang brown cattle
Effects of eight InDel variants in FHIT on milk traits in Xinjiang brown cattle
复制标题
FHIT中8个InDel变异对新疆褐牛牛奶性状的影响
DOI：
10.1080/10495398.2020.1724124
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发表时间：
2020-05-11
影响因子：
3.7
通讯作者：
Jiang，Hui
中科院分区：
文献类型：
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作者：
Ju，Xing;Huang，Xixia;Jiang，Hui
摘要在我们前期的全基因组关联研究（GWAS）中，我们发现新疆褐牛（XJBC）的脆性组氨酸三联体二腺苷三磷酸酶（FHIT）基因是与牛生产性状相关的候选基因，在牛育种的标记辅助选择（MAS）中具有潜在的应用价值。FHIT是一类肿瘤抑制基因的原型，其包含定位到常见脆性基因座的基因组基因座。在这里，388健康和无关的XJBC被选中，以确定插入/缺失（InDel）的变体在牛FHIT和评估其对牛奶性状的影响。13个InDel位点中有8个在FHIT中呈多态性。8个位点的多态信息含量在0.061 ~ 0.375之间。相关分析表明，所有新的InDel变异体与6个乳品质性状均呈显著相关（p < 0.05）。P2- 23 bp与305头奶牛的产奶量呈显著正相关（p = 0.005）第六胎; P3- 24 bp与乳脂产量（p = 0.009）; P5- 21 bp与体细胞评分（p = 0.001）在第一次产次和乳蛋白百分比P7- 26 bp与第6胎的体细胞评分（p = 0.003）。这些发现将有助于评估InDel基因型，牛品种内和品种之间，并确定潜在的目标基因座，以加快在牛育种MAS的进展。
Abstract In our previous genome-wide association study (GWAS), we identified the fragile histidine triad diadenosine triphosphatase (FHIT) gene in Xinjiang brown cattle (XJBC) as a candidate gene associated with cattle productive traits, with potential application in mark-assisted selection (MAS) in cattle breeding. FHIT is a prototype of a class of tumor suppressor genes that contain genomic loci mapped to common fragile loci. Here, 388 healthy and unrelated XJBC were selected to identify insertion/deletion (InDel) variants in the bovine FHIT and assess their effects on milk traits. Eight of the thirteen InDel loci were found to be polymorphic in FHIT. The polymorphism information content of the eight loci ranged from 0.061 to 0.375. The correlation analysis showed that all the new InDel variants were significantly related to six different milk traits (p < 0.05). The following variants presented a significant relationship with productive traits: P2-23bp with the 305 milk yield (p = 0.005) in the sixth parity; P3-24bp with the milk fat yield (p = 0.009) in the third parity; P5-21bp with the somatic cell score (p = 0.001) in the first parity and with the milk protein percentage (p = 0.002) in the sixth parity; and P7-26bp with the somatic cell score (p = 0.003) in the sixth parity. These findings will help evaluate InDel genotypes, within and between cattle breeds and identify potential target loci to accelerate progress in MAS in cattle breeding.