NONMUSCLE AND SMOOTH-MUSCLE MYOSIN ISOFORMS IN BOVINE ENDOTHELIAL-CELLS
NONMUSCLE AND SMOOTH-MUSCLE MYOSIN ISOFORMS IN BOVINE ENDOTHELIAL-CELLS
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DOI：
10.1016/0014-4827(90)90136-x
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发表时间：
1990-09-01
影响因子：
3.7
通讯作者：
SARTORE, S
中科院分区：
文献类型：
--
作者：
BORRIONE, AC;ZANELLATO, AMC;SARTORE, S
用一组特异性抗人血小板（NM-A9、NM-F6和NM-G2）和抗牛平滑肌（SM-E7）肌球蛋白重链（MHC）的单克隆抗体，在体内和体外研究牛内皮细胞（EC）中肌球蛋白亚型的组成和分布。利用间接和双重免疫荧光技术，我们发现，在完整的主动脉内皮细胞有非肌肉MHC（NM-MHC）的表达，排他。相比之下，肝窦内皮细胞以及培养的牛主动脉EC（BAEC）在生长的亚融合期显示NM-和平滑肌MHC（SM-MHC）亚型的共存。SM肌球蛋白免疫反应在培养的BAEC融合时消失。在细胞生长的这一阶段，NM-MHC同种型在细胞内的定位不同，即，在细胞核周围的细胞质中或在EC细胞质的皮层、膜下区域中。第三种类型的细胞内分布的NM-MHC免疫反应是明显的双核，融合BAEC的细胞周边。这些数据表明：（1）几种肌球蛋白异构体在牛内皮细胞中的分布不同;（2）SM肌球蛋白表达和NM肌球蛋白异构体在EC中的特异性亚细胞定位可能受到细胞-细胞相互作用的调节。
A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluorescence techniques, we have found that in the intact aortic endothelium there is expression of nonmuscle MHC (NM-MHC), exclusively. By contrast, hepatic sinusoidal endothelium as well as cultured bovine aortic EC (BAEC) in the subconfluent phase of growth show coexistence of NM- and smooth muscle MHC (SM-MHC) isoforms. SM myosin immunoreactivity disappears when cultured BAEC become confluent. In this phase of cell growth, NM-MHC isoforms are localized differently within the cells, i.e., in the cytoplasm around the nucleus or in the cortical, submembranous region of EC cytoplasm. A third type of intracellular distribution of NM-MHC immunoreactivity was evident in the cell periphery of binucleated, confluent BAEC. These data indicate that (1) several myosin isoforms are differently distributed in bovine endothelia; and (2) SM myosin expression and the specific subcellular localization of NM myosin isoforms within EC might be regulated by cell-cell interactions.