Controlled-synthesis of alumina-graphene oxide nanocomposite coupled with DNA/ sulfide fluorophore for eco-friendly "Turn off/on" H2S nanobiosensor.
Controlled-synthesis of alumina-graphene oxide nanocomposite coupled with DNA/ sulfide fluorophore for eco-friendly "Turn off/on" H2S nanobiosensor.
复制标题
DOI：
10.1016/j.talanta.2019.120655
复制
发表时间：
2020-05
期刊：
影响因子：
6.1
通讯作者：
Nadia A. Samak;M. Selim;Zhifeng Hao;J. Xing
中科院分区：
文献类型：
--
作者：
Nadia A. Samak;M. Selim;Zhifeng Hao;J. Xing
硫化氢(H_2S)是一种有毒的环境污染物，是人体生理的重要气体信号剂。迫切需要开发一种灵敏和选择性的硫化氢检测方法。为此，发展了一种新的关断/开启响应方法，将γ-Al_2O_3纳米棒固定在氧化石墨烯(GO)纳米片上，并与DNA/硫化物荧光团(SF)相结合，在室温下检测H_2S。荧光基团DNA/SF在γ-Al_2O_3-GO超强猝灭剂表面上保持猝灭状态。在有H_2S存在的情况下，由于H_2S分子与γ-Al_2O_3-GO杂化表面之间存在较强的物理吸附作用，γ-Al_2O_3-GO杂化表面上的DNA/SF荧光团发生了分离。恢复的荧光强度直接反映了介质中H_2S的浓度。所研制的γ-Al_2O_3-GO/DNA/SF纳米生物传感器对H_2S具有较高的灵敏度，对H_2S的线性检测范围为75~2.5μM，结合常数高达9.8×10~3M−_1。该传感器在各种干扰阴离子和硫醇存在下对H_2S具有很高的选择性，用于实际水样中H_2S的检测。γ-Al_2O_3-GO/DNA/SF纳米生物传感器提供了一种廉价、简便、高选择性的常温下检测H_2S的方法。
Hydrogen sulfide (H2S) is a toxic environmental pollutant and crucial gas-signaling agent of human physiology. There is an urgent need for developing a sensitive and selective detection approach for H2S. Herein, a novel turn off/on response approach using γ-Al2O3nanorods anchored on the surface graphene oxide (GO) nanosheets and coupled with DNA/sulfide fluorophore (SF) for H2S detection at room temperature was developed. The fluorescent fluorophore, DNA/SF, remains quenched on the super quencher γ-Al2O3–GO hybrid surface. In the existence of H2S, DNA/SF fluorophore was detached from γ-Al2O3–GO hybrid surface because of the strong physical adsorption interaction between H2S molecules and γ-Al2O3–GO surface. The recovered fluorescence intensity gave direct insight about H2S concentration in the medium. The developed γ-Al2O3–GO/DNA/SF nanobiosensor shows high sensitivity with 75 to 2.5 μM linear detection range of H2S, and a high binding constant of 9.8 × 103M−1. The nanobiosensor is very selective toward H2S in the presence of various interfering anions and thiols and used for H2S detection in real water samples. γ-Al2O3–GO/DNA/SF nanobiosensor provides a cheap, simple, and highly selective H2S detection method at room temperature.