Characterization of three human oligodendroglial cell lines as a model to study oligodendrocyte injury: Morphology and oligodendrocyte-specific gene expression
Characterization of three human oligodendroglial cell lines as a model to study oligodendrocyte injury: Morphology and oligodendrocyte-specific gene expression
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DOI：
10.1023/a:1027324230923
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发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Steels, P
中科院分区：
文献类型：
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作者：
Buntinx, M;Vanderlocht, J;Steels, P
少突胶质细胞是中枢神经系统的髓鞘形成细胞，是多发性硬化症中致病性免疫反应的靶点。人少突胶质细胞的原代培养已被用于阐明免疫介导的少突胶质细胞损伤的细胞和分子机制。然而，这些研究受到有限的可用性活人脑组织的阻碍。本研究旨在比较人少突胶质细胞系HOG、MO3.13和KG-1C的形态学和生化特性。我们已经确定了这些线的少突胶质细胞相关的功能，并分析了在何种程度上，他们可以被用来作为一个模型的人少突胶质细胞在特定的发育阶段被捕。少突胶质细胞系都表现出不成熟的少突胶质细胞的标记物，如CN β和GalC，但没有星形胶质细胞标记物GFAP。HOG和MO3.13细胞可诱导分化，如通过增殖减少、过程延伸增加而不形成髓鞘片和与成熟少突胶质细胞相关的基因如MBP和MOG的上调所见。基因芯片分析显示HOG细胞中有MAG、MOBP和OMG基因的表达。KG-1C细胞在推荐的条件下表现出较差的生长特性。总之，我们的数据表明，少突胶质细胞系HOG和MO3.13可以被用作一个模型的人少突胶质细胞'逮捕'在一个不成熟的发育阶段。在适当的培养基中培养可以诱导这些细胞的进一步分化。因此，这些细胞系可用作研究与疾病相关的免疫介导的少突胶质细胞损伤的模型。
Oligodendrocytes, the myelin-forming cells of the central nervous system, are the target of pathogenic immune responses in multiple sclerosis. Primary cultures of human oligodendrocytes have been used to unravel the cellular and molecular mechanisms of immune-mediated injury of oligodendrocytes. However, these studies are hampered by the limited availability of viable human brain tissue. The present study was aimed at comparing the morphological and biochemical characteristics of the human oligodendroglial cell lines HOG, MO3.13 and KG-1C. We have determined oligodendrocyte-associated features of these lines and analyzed the degree to which they can be used as a model of human oligodendrocytes arrested at specific developmental stages. The oligodendroglial cell lines all exhibited markers of immature oligodendrocytes, such as CNPase and GalC, but not the astrocytic marker GFAP. Differentiation could be induced in HOG and MO3.13 cells, as was seen through a decrease in proliferation, an increase in process extension without formation of myelin sheets and up-regulation of genes associated with mature oligodendrocytes such as MBP and MOG. Microarray analysis revealed the expression of MAG, MOBP and OMG genes in HOG cells. The KG-1C cells displayed poor growth characteristics in the recommended conditions. In conclusion, our data show that the oligodendroglial cell lines HOG and MO3.13 can be used as a model of human oligodendrocytes 'arrested' in an immature developmental stage. Culturing in appropriate medium can induce further differentiation of these cells. These cell lines can therefore be applied as a model to study immune-mediated injury of oligodendrocytes in relation to disease.