Extended MLST-based population genetics and phylogeny of Vibrio parahaemolyticus with high levels of recombination
Extended MLST-based population genetics and phylogeny of Vibrio parahaemolyticus with high levels of recombination
复制标题
具有高重组水平的副溶血性弧菌基于 MLST 的扩展群体遗传学和系统发育。
DOI：
10.1016/j.ijfoodmicro.2010.11.038
复制
发表时间：
2011-01-31
影响因子：
5.4
通讯作者：
Zhou, Dongsheng
中科院分区：
文献类型：
--
作者：
Yan, Yanfeng;Cui, Yujun;Zhou, Dongsheng
基于 10 个保守基因，通过多位点序列分型 (MLST) 对 174 个副溶血弧菌全球分离株进行了分析。结果显示出高水平的核苷酸和等位基因多样性，并有纯化选择和频繁重组的证据。重组在产生遗传异质性方面比突变发挥更大的作用。 174 个菌株可分为 89 个不同的序列类型，这些序列类型可进一步分为 6 个克隆复合体（CC；CC1 至 CC6）以及 71 个单克隆。三个主要CC，即CC1至CC3，对应于1996年之前临床旧O3组：K6毒株（trh（+），T3SS2 beta（+），tdh（-），T3SS2 alpha（-）和GS-PCR-），1996年后大流行毒株（trh（-），T3SS2 beta（-），tdh（+），T3SS2 α(+) 和 GS-PCR+) 和非临床分离株(trh(-)、T3SS2 β(-)、tdh(-)、T3SS2 α(-) 和 GS-PCR-)。 MLST数据能够从等位基因谱而不是核苷酸序列构建系统发育结构，从而减少频繁重组的影响。这六个CC是在突变和重组的背景下产生的，根据之前报道的数据，由于外源基因的横向获得，特别是包括编码毒力决定子的基因，该细菌可以快速进化。副溶血弧菌具有典型的流行种群结构，由突变、重组和横向基因转移驱动。 (c) 2010 Elsevier B.V. 保留所有权利。
A collection of 174 global isolates of Vibrio parahaemolyticus were analyzed by multilocus sequence typing (MLST) on the basis of ten conserved genes. The results showed a high level of nucleotide and allelic diversity with the evidence of purifying selection and of frequent recombination. Recombination played a much greater role than mutation in generating genetic heterogeneity. The 174 strains could be assigned into 89 different sequence types, which could be further separated into six clonal complexes (CCs; CC1 to CC6) plus 71 singletons. The three major CCs, namely CC1 to CC3, corresponded to the groups of pre-1996 clinical old-O3: K6 strains (trh(+), T3SS2 beta(+), tdh(-), T3SS2 alpha(-), and GS-PCR-), post-1996 pandemic strains (trh(-), T3SS2 beta(-), tdh(+), T3SS2 alpha(+), and GS-PCR+) and non-clinical isolates (trh(-), T3SS2 beta(-), tdh(-), T3SS2 alpha(-), and GS-PCR-), respectively. The MLST data enable the construction of a phylogenetic structure from the allelic profiles rather the nucleotide sequences, so as to reduce the affect of frequent recombination. The six CCs arose on a background of mutation and recombination, and according to the previously reported data, this bacterium could be evolved fast due to lateral acquisition of foreign genes especially including those encoding virulence determinants. V. parahaemolyticus had a typical epidemic population structure that is driven by mutation, recombination and lateral gene transfer. (c) 2010 Elsevier B.V. All rights reserved.