Photodynamic Physiology-Photonanomanipulations in Cellular Physiology with Protein Photosensitizers.
Photodynamic Physiology-Photonanomanipulations in Cellular Physiology with Protein Photosensitizers.
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光动力生理学 - 使用蛋白质光敏剂进行细胞生理学中的光纳米操作
DOI：
10.3389/fphys.2017.00191
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发表时间：
2017
影响因子：
4
通讯作者：
Cui ZJ
中科院分区：
文献类型：
--
作者：
Jiang HN;Li Y;Cui ZJ
在II型光动力作用中产生的单线态氧，由于其有限的寿命（1 μs）和反应距离（<10 nm），可以在纳米尺度上调节活细胞的功能。基因编码的蛋白质光敏剂（工程化荧光蛋白如KillerRed、TagRFP和黄素结合蛋白如miniSOG、Pp 2FbFPL 30 M）可以以细胞类型和/或亚细胞器特异性方式表达，用于靶向蛋白质光氧化活化/脱敏。新出现的主动照明技术提供了额外的特异性水平。光动力活化的典型实例包括G蛋白偶联受体CCK 1的永久活化和离子通道TRPA 1的光动力活化。蛋白质光敏剂已被用于光动力学调节主要的细胞功能（如神经递质释放和基因转录）和动物行为。蛋白质光敏剂越来越多地用于细胞生理学研究中的光子驱动的纳米操纵。
Singlet oxygen generated in a type II photodynamic action, due to its limited lifetime (1 μs) and reactive distance (<10 nm), could regulate live cell function nanoscopically. The genetically-encoded protein photosensitizers (engineered fluorescent proteins such as KillerRed, TagRFP, and flavin-binding proteins such as miniSOG, Pp2FbFPL30M) could be expressed in a cell type- and/or subcellular organelle-specific manner for targeted protein photo-oxidative activation/desensitization. The newly emerged active illumination technique provides an additional level of specificity. Typical examples of photodynamic activation include permanent activation of G protein-coupled receptor CCK1 and photodynamic activation of ionic channel TRPA1. Protein photosensitizers have been used to photodynamically modulate major cellular functions (such as neurotransmitter release and gene transcription) and animal behavior. Protein photosensitizers are increasingly used in photon-driven nanomanipulation in cell physiology research.
细胞内部的Killerr的定位效应与可见光激活后与DNA链断裂相关。
影响因子：
3.6
作者：
Waldeck W;Mueller G;Wiessler M;Tóth K;Braun K
通讯作者：
Braun K