Oncostatin M stimulates proliferation and functions of mouse fetal liver cells in three-dimensional cultures
Oncostatin M stimulates proliferation and functions of mouse fetal liver cells in three-dimensional cultures
复制标题
DOI：
10.1002/jcp.20167
复制
发表时间：
2005-03-01
影响因子：
5.6
通讯作者：
Ohshima, N
中科院分区：
文献类型：
--
作者：
Ehashi, T;Miyoshi, H;Ohshima, N
为了开发组织工程化生物人工肝（BAL），利用多孔聚合物作为支架进行胎肝细胞（FLC）的长期三维（3-D）培养长达1个月。在三维培养和传统单层培养皿中，研究了基础培养基和添加抑瘤素M（OSM）对小鼠FLC增殖和分化的影响。与单层培养相比，三维培养能更好地维持FLC的细胞数量和肝功能。当在本研究中测试两种基础培养基时，威廉姆斯培养基E（WE）在表达FLC在3-D和单层培养中的肝功能方面上级最低必需培养基α（alphaMEM），尽管用alphaMEM获得更高的细胞密度。OSM有效地刺激细胞生长和代谢活性，特别是在三维培养中。当添加OSM的WE用于3-D培养时，FLC的白蛋白分泌在第5天后显著增加，并且高水平的分泌维持到培养结束。在超过1个月的时间内，未观察到白蛋白分泌减少。总之，这种三维培养方法有望成为开发组织工程化BAL的现实尝试之一。J.细胞。(C)2004年威利-利斯，hic。
in order to develop a tissue engineered bioartificial liver (BAL), long-term three-dimensional (3-D) culture of fetal liver cells (FLCs) utilizing porous polymer as a scaffold was performed for up to I month. The effects of the basal medium and supplementation with oncostatin M (OSM) on the proliferation and differentiation of mouse FLCs were examined in both 3-D culture and conventional monolayer dish culture. Compared with monolayer culture, cell numbers and hepatic function of FLCs were better maintained by 3D culture. When two kinds of basal media were tested in this study, Williams' medium E (WE) was superior to minimum essential medium alpha (alphaMEM) in expressing hepatic function of FLCs in both 3-D and monolayer cultures, although higher cell densities were obtained with alphaMEM. OSM potently stimulated both cell growth and metabolic activity, especially in 3-D culture. When WE supplemented with OSM was used for 3-D culture, albumin secretion by FLCs increased dramatically after day 5, and a high level of secretion was maintained until the end of culture. During a period of over I month, no decrease of albumin secretion was observed. In conclusion, this 3-D culture method was expected to be one of the realistic attempts to develop a tissue engineered BAL. J. Cell. (C) 2004 Wiley-Liss, hic.