Lipid arrangement in fluid model membranes: analysis by cross-linking of phosphatidylethanolamines.
Lipid arrangement in fluid model membranes: analysis by cross-linking of phosphatidylethanolamines.
复制标题
流体模型膜中的脂质排列：通过磷脂酰乙醇胺的交联进行分析。
DOI：
10.1016/0005-2736(91)90377-k
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Welti,R
中科院分区：
文献类型：
--
作者：
Roth,MR;Welti,R
用双官能团交联剂二甲基磺酰亚胺处理了pH为10的磷脂酰乙醇胺二元液相混合物。对形成的二聚体物种的分析表明，在52℃时，二聚磷脂酰乙醇胺/二烯丙酰基磷脂酰乙醇胺混合物中的磷脂物种是随机排列的；在41℃时，二烯丙酰基磷脂酰乙醇胺/二月桂酰基磷脂酰乙醇胺混合物中的磷脂物种是随机排列的。对二棕榈酰磷脂酰乙醇胺/二油酰磷脂酰乙醇胺混合物在68℃下形成的二聚体物种的分析表明，该混合物非常接近随机排列，只是有轻微的相似磷脂物种聚集的倾向。
Binary mixtures of fluid phase phosphatidylethanolamines at pH 10 were treated with the bifunctional cross-linking reagent dimethylsuberimidate. Analysis of the dimeric species formed demonstrated that the phospholipid species in dimyristoylphosphatidylethanolamine / dielaidoylphosphatidylethanolamine mixtures at 52°C and dielaidoylphosphatidylethanolamine / dilauroylphosphatidylethanolamine mixtures at 41° C were randomly arranged. Analysis of the dimeric species formed in dipalmitoylphosphatidylethanolamine / dioleoylphosphatidylethanolamine mixtures at 68°C showed that this mixture was very close to being randomly arranged, with just a slight propensity of like phospholipid species to cluster.