StGA2ox1 is induced prior to stolon swelling and controls GA levels during potato tuber development
StGA2ox1 is induced prior to stolon swelling and controls GA levels during potato tuber development
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DOI：
10.1111/j.1365-313x.2007.03245.x
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发表时间：
2007-10-01
期刊：
影响因子：
7.2
通讯作者：
Bachem, Christian W. B.
中科院分区：
文献类型：
--
作者：
Kloosterman, Bjorn;Navarro, Christina;Bachem, Christian W. B.
马铃薯块茎的形成和生长是一个复杂的过程，受不同环境信号和植物激素的调控。特别地，赤霉素（GAs）的作用涉及马铃薯块茎形成的不同方面。在这里，我们报告的分离和功能分析的马铃薯GA 2-氧化酶基因（StGA 2 ox 1）及其在块茎形成中的作用。StGA 2 ox 1在马铃薯块茎发育的早期阶段可见肿胀之前上调，主要在匍匐茎和生长块茎的亚顶端区域表达。35 S-过表达转化体表现出矮化表型，匍匐茎生长减少和早期的试管块茎化。StGA 2 ox 1的表达水平降低的转基因植物表现出正常的植物生长，改变匍匐茎肿胀表型和延迟在体外结瘤。StGA 2 ox 1抑制克隆的块茎含有增加水平的GA（20），表明GA代谢改变。我们提出了一个角色StGA 2 ox 1在早期块茎启动修改GA水平在茎尖下匍匐茎区域在块茎化的发病，从而促进正常的块茎发育和生长。
The formation and growth of a potato (Solanum tuberosum) tuber is a complex process regulated by different environmental signals and plant hormones. In particular, the action of gibberellins (GAs) has been implicated in different aspects of potato tuber formation. Here we report on the isolation and functional analysis of a potato GA 2-oxidase gene (StGA2ox1) and its role in tuber formation. StGA2ox1 is upregulated during the early stages of potato tuber development prior to visible swelling and is predominantly expressed in the subapical region of the stolon and growing tuber. 35S-over-expression transformants exhibit a dwarf phenotype, reduced stolon growth and earlier in vitro tuberization. Transgenic plants with reduced expression levels of StGA2ox1 showed normal plant growth, an altered stolon swelling phenotype and delayed in vitro tuberization. Tubers of the StGA2ox1 suppression clones contain increased levels of GA(20), indicating altered GA metabolism. We propose a role for StGA2ox1 in early tuber initiation by modifying GA levels in the subapical stolon region at the onset of tuberization, thereby facilitating normal tuber development and growth.