Vapour-mediated ion activation for enhanced SIMS imaging
Vapour-mediated ion activation for enhanced SIMS imaging
复制标题
用于增强 SIMS 成像的蒸汽介导离子激活
DOI：
10.1002/sia.4883
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发表时间：
2012
影响因子：
1.7
通讯作者：
Vaidyanathan S
中科院分区：
文献类型：
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作者：
Vaidyanathan S
飞行时间二次离子质谱（ToF - SIMS）成像的成功取决于被成像样品表面分析物的有效溅射和电离。尽管由于簇离子束的出现，SIMS分析已经取得了重大进展，但这主要是在溅射过程中。电离在很大程度上仍然是无效的，在这个方向上的改进将显著增强SIMS成像。我们特别感兴趣的是提高SIMS成像检测生物细胞和组织中代谢物（代谢的小分子量成分）的能力。在这里，我们报告了一种方法来增强诊断离子的信号强度，并改善使用蒸汽介导的离子激活方法代谢物的SIMS成像。将代谢物的合成鸡尾酒暴露于氨蒸气中，表明鸡尾酒中某些代谢物的去质子化离子信号增加了三倍以上。然而，室温暴露导致样品形态的变化，表明样品表面被破坏。当在4°C下进行处理时，可以提高离子产率并保持样品的形貌。将该方法应用于植物组织切片显示了未处理样品中未观察到的信号的检测。这是一种很有前途的方法，可用于增强对有机和生物样品的ToF - SIMS分析的诊断离子的检测。版权所有©2012 John Wiley & Sons， Ltd。
The success of time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) imaging is dependent on effective sputtering and ionisation of analytes from the sample surface imaged. Although there have been significant advances in SIMS analysis, thanks to the advent of cluster ion beams, this has largely been in the sputtering process. Ionisation is still largely ineffective, and improvements in this direction would significantly enhance SIMS imaging. We are specifically interested in improving the capabilities of SIMS imaging for detecting metabolites (small molecular weight components of metabolism) in biological cells and tissues. Here, we report a method to enhance the signal intensities of diagnostic ions and improve SIMS imaging of metabolites using a vapour‐mediated ion activation approach. Exposure of a synthetic cocktail of metabolites to ammonia vapour showed a greater than threefold increase in the deprotonated ion signals for some of the metabolites in the cocktail. However, room temperature exposure resulted in changes to the sample morphology, indicating that the sample surface was disrupted. It was possible to increase the ion yield and retain the sample morphology when the treatment was carried out at 4 °C. Application of the methodology to a plant tissue section showed the detection of signals that were not observed in the samples that were not treated. This is a promising approach that can be applied to enhance the detection of diagnostic ions for the ToF‐SIMS analysis of organic and biological samples. Copyright © 2012 John Wiley & Sons, Ltd.