Iowa State University From the SelectedWorks of Steven Whitham 1999 Selectable viruses and altered susceptibility mutants in Arabidopsis thaliana
Iowa State University From the SelectedWorks of Steven Whitham 1999 Selectable viruses and altered susceptibility mutants in Arabidopsis thaliana
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发表时间：
2017
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通讯作者：
Steven;-A.;Whitham;Miki;-L.;Yamamoto;James;-C.;Carrington
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作者：
Steven;-A.;Whitham;Miki;-L.;Yamamoto;James;-C.;Carrington
人们对植物对病毒敏感或不敏感的遗传基础知之甚少。开发了两种选择性烟草蚀纹病毒（TEV）菌株，用于鉴定具有易感性增加或易感性丧失表型的拟南芥突变体。这些菌株基于除草剂抗性或除草剂敏感性基因的系统表达，赋予拟南芥有条件生存表型，从而促进大规模选择和筛选增强或抑制 TEV 复制、细胞间运动或长距离运动的拟南芥突变体。通过分离具有改变的易感性突变体，确定了限制 TEV 系统性侵袭的多组分机制。
The genetic basis for susceptibility or nonsusceptibility of plants to viruses is understood poorly. Two selectable tobacco etch virus (TEV) strains were developed for identification of Arabidopsis thaliana mutants with either gain-of-susceptibility or loss-of-susceptibility phenotypes. These strains conferred a conditional-survival phenotype to Arabidopsis based on systemic expression of herbicide resistance or proherbicide sensitivity genes, thereby facilitating mass selections and screens for Arabidopsis mutants that enhance or suppress TEV replication, cell-to-cell movement, or long-distance movement. A multicomponent mechanism that restricts systemic invasion of TEV was identified through isolation of gain-of-susceptibility mutants with alterations at