Effects of Storage Conditions of Bovine Ovaries and Oocytes on the Success Rate of in Vitro Fertilization and Culture
Effects of Storage Conditions of Bovine Ovaries and Oocytes on the Success Rate of in Vitro Fertilization and Culture
复制标题
牛卵巢和卵母细胞保存条件对体外受精培养成功率的影响
DOI：
10.1262/jrd.38.11
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发表时间：
1992
影响因子：
1.8
通讯作者：
N. Nakatsuji
中科院分区：
文献类型：
--
作者：
H. Nakao;N. Nakatsuji
本研究探讨了牛卵巢和/或卵母细胞的保存条件对受精卵体外受精(IVF)和随后的体外培养(IVC)成功率的影响。从屠宰场获得的卵巢保存在39℃(第1组)、20℃(第2组)或4℃(第3组)的林格液中。另一组从卵巢中分离出未成熟卵母细胞，保存在39℃的Tcm-199培养液中(第4组)。在屠宰场，从屠宰开始，整个卵巢收集程序大约需要3小时。实验前将卵巢和/或卵母细胞在上述条件下保存5h。对照组从卵巢中分离卵母细胞，立即培养，诱导成熟。经体外受精和体外受精后，组1和组3的受精卵均未发育到桑拿期。组2的受精卵发育到桑囊期和囊胚期的比例分别为11%和7%，但显著低于对照组(32%和15%)。第4组的成功率(38%，13%)与对照组相似。
The present study was carried out to examine effects of storage conditions of bovine ovaries and/or oocytes on the success rate of in vitro fertilization (IVF) and subsequent in vitroculture (IVC) of the zygotes. Ovaries obtained from a slaughter house were stored in Ringer solution at 39C (group 1), 20C (group 2), or 4C (group 3). In another group, immature oocytes were isolated from ovaries and stored in TCM-199 medium at 39C (group 4). The entire collection procedures of the ovaries took approximately 3 h at the slaughter house from the time of slaughter. The ovaries and/or the oocytes were stored under the conditions described above for 5 h before the experiment. In the control group, oocytes were isolated from the ovaries and cultured immediately to induce maturation. Following IVF and IVC, none of the zygotes developed to morula stage in group 1 and 3. In group 2, some zygotes developed to morula or blastocyst stage (11%, 7% respectively), but the rates were significantly lower than in the control group (32%, 15%). Group 4 gave high success rates (38%, 13%) similar to those attained in the control gorup.