Protective role of mitochondrial superoxide dismutase against high osmolarity, heat and metalloid stress in Saccharomyces cerevisiae
Protective role of mitochondrial superoxide dismutase against high osmolarity, heat and metalloid stress in Saccharomyces cerevisiae
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DOI：
10.1007/bf02932150
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发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Sigler, K.
中科院分区：
文献类型：
--
作者：
Dziadkowiec, D.;Krasowska, A.;Sigler, K.
超氧化物歧化酶（SOD1 编码的胞质 Cu,Zn-SOD 和 SOD2 编码的线粒体 Mn-SOD）可帮助酿酒酵母细胞防御超氧自由基，但 sod1 Delta 和 sod2 Delta 突变株的表型不同。与亲本菌株和 sod1 Delta 突变体相比，sod2 Delta 突变体在高盐浓度下表现出更严重的生长缺陷，但 2 mmol/L 谷胱甘肽可部分挽救这种缺陷。所有三种菌株的生长在 37°C 时都会减少，sod2 Delta 显示出最高的敏感性，特别是在空气中培养时。在培养基中添加 1 mmol/L 谷胱甘肽可恢复 sod1 Delta 突变体的有氧生长，但对 sod2 Delta 菌株在 37 ℃ 下的生长影响很小。 sod2 Delta 菌株对 As-III 和 As-V 也敏感，并且其敏感性在有氧条件下更为明显。这些结果表明，与主要作用是氧化应激防御的 Sod1p 蛋白不同，Sod2p 还在保护酿酒酵母细胞免受其他应激（高渗透压、热和非金属应激）方面发挥作用。
Superoxide dismutases, both cytosolic Cu,Zn-SOD encoded by SOD1 and mitochondrial Mn-SOD encoded by SOD2, serve Saccharomyces cerevisiae cells for defense against the superoxide radical but the phenotypes of sod1 Delta and sod2 Delta mutant strains are different. Compared with the parent strain and the sod1 Delta mutant, the sod2 Delta mutant shows a much more severe growth defect at elevated salt concentrations, which is partially rescued by 2 mmol/L glutathione. The growth of all three strains is reduced at 37 degrees C, the sod2 Delta showing the highest sensitivity, especially when cultured in air. Addition of 1 mmol/L glutathione to the medium restores aerobic growth of the sod1 Delta mutant but has only a minor effect on the growth of the sod2 Delta strain at 37 degrees C. The sod2 Delta strain is also sensitive to As-III and As-V and its sensitivity is much more pronounced under aerobic conditions. These results suggest that, unlike the Sod1p protein, whose major role is oxidative stress defense, Sod2p also plays a role in protecting S. cerevisiae cells against other stresses - high osmolarity, heat and metalloid stress.