DETECTION OF CHROMOSOME-ABERRATIONS IN METAPHASE AND INTERPHASE TUMOR-CELLS BY INSITU HYBRIDIZATION USING CHROMOSOME-SPECIFIC LIBRARY PROBES
DETECTION OF CHROMOSOME-ABERRATIONS IN METAPHASE AND INTERPHASE TUMOR-CELLS BY INSITU HYBRIDIZATION USING CHROMOSOME-SPECIFIC LIBRARY PROBES
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DOI：
10.1007/bf01790091
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发表时间：
1988-11-01
期刊：
影响因子：
5.3
通讯作者：
MANUELIDIS, L
中科院分区：
文献类型：
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作者：
CREMER, T;LICHTER, P;MANUELIDIS, L
使用生物素化 DNA 文库探针分析了两种神经胶质瘤细胞系中的染色体畸变，这些探针特异性地装饰 1、4、7、18 和 22 号染色体从 pter 到 qter。这些染色体的数值变化、缺失和重排在中期扩散以及早期前期和间期细胞核中很容易观察到。完整的染色体、缺失的染色体和易位染色体片段被快速描绘成非常复杂的核型。与额外的亚区域探针的同时杂交被用来进一步定义异常染色体。使用数字图像分析来定量每个细胞系的各个中期和间期细胞中特定染色体 DNA 的总补体。尽管这两种神经胶质瘤系已在体外传代多年，但仍观察到 22 号染色体代表性不足，而 7 号染色体（特别是 7p）代表性过度。这些观察结果与之前关于神经胶质瘤的研究一致。此外，还发现 4 号染色体的序列代表性不足，尤其是在 TC 593 中。这些分析表明这些方法在精确定位特定类型肿瘤中发生改变的染色体片段方面的能力。
Chromosome aberrations in two glioma cell lines were analyzed using biotinylated DNA library probes that specifically decorate chromosomes 1, 4, 7, 18 and 22 from pter to qter. Numerical changes, deletions and rearrangements of these chromosomes were readily visualized in metaphase spreads, as well as in early prophase and interphase nuclei. Complete chromosomes, deleted chromosomes and segments of translocated chromosomes were rapidly delineated in very complex karyotypes. Simultaneous hybridizations with additional subregional probes were used to further define aberrant chromosomes. Digital image analysis was used to quantitate the total complement of specific chromosomal DNAs in individual metaphase and interphase cells of each cell line. In spite of the fact that both glioma lines have been passaged in vitro for many years, an under-representation of chromosome 22 and an over-representation of chromosome 7 (specifically 7p) were observed. These observations agree with previous studies on gliomas. In addition, sequences of chromosome 4 were also found to be under-represented, especially in TC 593. These analyses indicate the power of these methods for pinpointing chromosome segments that are altered in specific types of tumors.