Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians
Transcriptomic analysis reveals differences in the regulation of amino acid metabolism in asexual and sexual planarians
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DOI：
10.1038/s41598-019-42025-z
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发表时间：
2019-04
影响因子：
4.6
通讯作者：
Kiyono Sekii;Shunta Yorimoto;Hikaru Okamoto;Nanna Nagao;T. Maezawa;Y. Matsui;K. Yamaguchi;Ryohei Furu
中科院分区：
文献类型：
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作者：
Kiyono Sekii;Shunta Yorimoto;Hikaru Okamoto;Nanna Nagao;T. Maezawa;Y. Matsui;K. Yamaguchi;Ryohei Furu
许多扁形虫可以在无性繁殖和有性繁殖之间交替。这是一种强大的繁殖策略，使它们能够受益于两种繁殖模式的特点，即快速繁殖和遗传洗牌。这两种生殖模式是通过多能成体干细胞（neoblast）的存在，通过在无性模式中产生任何类型的组织，以及在有性模式中产生和维持生殖细胞来实现的。本研究利用RNA测序技术（RNA-seq）比较了琉球三角涡虫（Dugesia ryukyuensis）两种表型的转录组：无性型OH株和实验性化的OH株。途径富集分析显示两种蠕虫的氨基酸代谢存在显著差异。此外，分析确定5-羟色胺作为一种新的生物活性物质，诱导涡虫ovaryde novoin胚后的方式。这些发现表明，不同的代谢状态和生理条件诱发的性诱导物质可能调节干细胞的行为，这取决于它们在无性和有性生殖模式的不同功能。RNA-seq和摄食试验的结合是研究琉球龙线虫繁殖模式交替、解开基因表达与化学信号分子之间关系的有力工具。
Many flatworms can alternate between asexual and sexual reproduction. This is a powerful reproductive strategy enabling them to benefit from the features of the two reproductive modes, namely, rapid multiplication and genetic shuffling. The two reproductive modes are enabled by the presence of pluripotent adult stem cells (neoblasts), by generating any type of tissue in the asexual mode, and producing and maintaining germ cells in the sexual mode. In the current study, RNA sequencing (RNA-seq) was used to compare the transcriptomes of two phenotypes of the planarianDugesia ryukyuensis: an asexual OH strain and an experimentally sexualized OH strain. Pathway enrichment analysis revealed striking differences in amino acid metabolism in the two worm types. Further, the analysis identified serotonin as a new bioactive substance that induced the planarian ovaryde novoin a postembryonic manner. These findings suggest that different metabolic states and physiological conditions evoked by sex-inducing substances likely modulate stem cell behavior, depending on their different function in the asexual and sexual reproductive modes. The combination of RNA-seq and a feeding assay inD. ryukyuensisis a powerful tool for studying the alternation of reproductive modes, disentangling the relationship between gene expression and chemical signaling molecules.