Large scale in vitro propagation of Stevia rebaudiana (bert) for commercial application: Pharmaceutically important and antidiabetic medicinal herb
Large scale in vitro propagation of Stevia rebaudiana (bert) for commercial application: Pharmaceutically important and antidiabetic medicinal herb
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DOI：
10.1016/j.indcrop.2011.10.037
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发表时间：
2012-05
影响因子：
5.9
通讯作者：
M. Thiyagarajan;P. Venkatachalam
中科院分区：
文献类型：
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作者：
M. Thiyagarajan;P. Venkatachalam
甜菊是一种珍贵的药用植物物种，用于治疗糖尿病。目前，由于人口中糖尿病疾病的不断增加，对这种草药的原料有很高的需求。为了满足日益增长的需求，研究了S. Rebaudiana成立了。从田间采集的节段外植体分别接种在附加不同浓度BAP（0.5-3.0mg/l）和KIN（0.5-3.0mg/l）的MS基本培养基上诱导芽。将离体节芽培养在添加不同浓度（0.5-3.0mg/l）BAP和KIN的MS培养基上，进行多芽再生。第二个试验是将离体芽接种在附加不同浓度BAP（0.5-3.0mg/l）的MS培养基上，再附加0.5mg/l的IAA或IBA或NAA进行芽增殖。在附加1.0mg/l BAP的MS培养基上，不定芽再生频率最高（94.50%），芽数最多（15.69个/外植体）。为了大规模生产，将离体茎芽外植体培养在附加1.0mg/l BAP的MS培养基上，在相同的培养基成分上继代3次后，每个外植体可获得约123个芽。从离体增殖的丛生芽中切取>2cm的伸长芽，培养在含不同浓度NAA（0.1-0.5mg/l）的半浓度MS培养基和/或添加不同浓度生长素（NAA、IAA和IBA）（0.5-2.0mg/l）的MS培养基上，诱导生根。生根率在MS + NAA0.4mg/l培养基上最高，达96%。生根苗成功地转移到装有1：2的砂和土壤的塑料杯中，随后在温室中建立。目前的体外繁殖协议将有助于快速和大规模生产这种重要的抗糖尿病药用植物的替代方法。
Stevia rebaudiana is a valuable medicinal plant species and it is being used for the treatment of diabetes. Currently, there is a high demand for raw material of this medicinal herb due to ever increasing diabetes disorder among the population. In order to meet the increased demand an efficient in vitro propagation of S. rebaudiana was established. Nodal explants collected from the field were cultured on MS basal medium fortified with different concentrations of BAP (0.5–3.0mg/l) and KIN (0.5–3.0mg/l) individually for shoot bud induction. In vitro derived nodal buds were cultured on MS medium supplemented with different concentrations (0.5–3.0mg/l) of BAP and KIN for multiple shoot bud regeneration. In the second experiment, in vitro derived buds were placed on MS medium supplemented with different concentrations of BAP (0.5–3.0mg/l) in combination with 0.5mg/l IAA or IBA or NAA for shoot bud multiplication. The highest frequency (94.50%) of multiple shoot regeneration with maximum number of shoots (15.69 shoots/explant) was noticed on MS medium supplemented with 1.0mg/l BAP. For large scale plant production, in vitro derived nodal bud explants were cultured on MS medium fortified with 1.0mg/l BAP, in which about 123 shoots/explant were obtained after three subcultures on the same media composition. Elongated shoots (>2cm) dissected out from the in vitro proliferated shoot clumps were cultured on half-strength MS medium containing different concentrations of NAA (0.1–0.5mg/l) and/or MS medium fortified with various concentrations (0.5–2.0mg/l) of auxins (NAA, IAA and IBA) for root induction. Highest frequency of rooting (96%) was noticed on half-strength MS medium augmented with 0.4mg/l NAA. The rooted plantlets were successfully transferred into plastic cups containing sand and soil in the ratio of 1:2 and subsequently established in the greenhouse. The present in vitro propagation protocol would facilitate an alternative method for rapid and large-scale production of this important antidiabetic medicinal plant.