Imaging of Liver Tumors Using Surface-Enhanced Raman Scattering Nanoparticles.
Imaging of Liver Tumors Using Surface-Enhanced Raman Scattering Nanoparticles.
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DOI：
10.1021/acsnano.5b07200
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发表时间：
2016-05-24
期刊：
影响因子：
17.1
通讯作者：
Kircher MF
中科院分区：
文献类型：
--
作者：
Andreou C;Neuschmelting V;Tschaharganeh DF;Huang CH;Oseledchyk A;Iacono P;Karabeber H;Colen RR;Mannelli L;Lowe SW;Kircher MF
完全手术切除是大多数肝脏恶性肿瘤的一线治疗方法。这一目标将通过术中成像方法来实现，该方法能够更精确地可视化肿瘤边缘，并检测否则不可见的显微病变。为此，我们合成了二氧化硅封装的表面增强拉曼散射（Sers）纳米粒子（NP），作为肝脏恶性肿瘤的分子成像剂。我们假设，静脉给药后，Sers纳米颗粒将热切地回家健康的肝组织，但不肝内恶性肿瘤。我们在肝细胞癌和组织细胞肉瘤的基因工程小鼠模型中测试了这些Sers纳米颗粒。静脉注射后，两种模型中的肝肿瘤均易于通过拉曼成像识别。此外，使用Sers NP的拉曼成像能够检测肝脏和脾脏中的显微病变。我们比较了Sers纳米颗粒的性能，使用吲哚菁绿色（ICG）的荧光成像。我们发现Sers纳米颗粒更准确地描绘肿瘤，并且对光漂白不太敏感。鉴于Sers成像的已知优点，即高灵敏度和特异性光谱检测，这些发现有望改善肝癌切除术。
Complete surgical resection is the first-line treatment for most liver malignancies. This goal would be facilitated by an intraoperative imaging method that enables more precise visualization of tumor margins, and detection of otherwise invisible microscopic lesions. To this end, we synthesized silica-encapsulated surface-enhanced Raman scattering (SERS) nanoparticles (NPs) that act as a molecular imaging agent for liver malignancies. We hypothesized that, after intravenous administration, SERS NPs would avidly home to healthy liver tissue, but not to intrahepatic malignancies. We tested these SERS NPs in genetically engineered mouse models of hepatocellular carcinoma and histiocytic sarcoma. After intravenous injection, liver tumors in both models were readily identifiable with Raman imaging. In addition, Raman imaging using SERS NPs enabled detection of microscopic lesions in liver and spleen. We compared the performance of SERS NPs to fluorescence imaging using Indocyanine Green (ICG). We found that SERS NPs delineate tumors more accurately and are less susceptible to photobleaching. Given the known advantages of SERS imaging, namely high sensitivity and specific spectroscopic detection, these findings hold promise for improved resection of liver cancer.