Urinary 1H-NMR metabolomics can distinguish pancreatitis patients from healthy controls.
Urinary 1H-NMR metabolomics can distinguish pancreatitis patients from healthy controls.
复制标题
DOI：
10.6092/1590-8577/1294
复制
发表时间：
2013-03-10
期刊：
影响因子：
--
通讯作者：
Conwell DL
中科院分区：
文献类型：
--
作者：
Lusczek ER;Paulo JA;Saltzman JR;Kadiyala V;Banks PA;Beilman G;Conwell DL
尿代谢物组的表征可以产生指示胰腺炎的生物标志物。我们建立了一种非侵入性的技术来比较急性和慢性胰腺炎患者与健康对照组的尿代谢谱。尿液取自健康对照组（HC，n=5）、轻度急性胰腺炎患者（AP，n=5）和慢性胰腺炎门诊患者（CP，n=5）。获得每个样品的质子核磁共振谱。在每个光谱中鉴定并定量代谢物;将所得浓度归一化以解释样品之间稀释度的差异。进行Kruskal-Wallis检验、事后Mann-Whitney U检验和主成分分析，以鉴定区分健康对照、急性胰腺炎和慢性胰腺炎的代谢物。鉴定并定量了60种代谢物;发现三组中有5种代谢物存在显着差异（P<0.05）。其中，柠檬酸盐和腺苷在通过随机排列验证后仍具有显著性。主成分分析表明，健康对照尿液样本可以区分慢性胰腺炎或急性胰腺炎患者;慢性胰腺炎患者不能区分急性胰腺炎患者。这项代谢组学研究表明，这种非侵入性技术提供了深入了解胰腺炎的代谢状态。虽然鉴定的代谢物不能最终被定义为疾病的生物标志物，但未来的研究将在更大的患者队列中验证我们的发现。
The characterization of the urinary metabolome may yield biomarkers indicative of pancreatitis. We establish a non-invasive technique to compare urinary metabolic profiles in patients with acute and chronic pancreatitis to healthy controls. Urine was obtained from healthy controls (HC, n=5), in patients with mild acute pancreatitis (AP, n=5), and outpatients with chronic pancreatitis (CP, n=5). Proton nuclear magnetic resonance spectra were obtained for each sample. Metabolites were identified and quantified in each spectrum; resulting concentrations were normalized to account for differences in dilution among samples. Kruskal-Wallis test, post-hoc Mann-Whitney U tests, and principal component analysis were performed to identify metabolites that discriminate healthy controls, acute pancreatitis, and chronic pancreatitis. Sixty metabolites were identified and quantified; five were found to differ significantly (P<0.05) among the three groups. Of these, citrate and adenosine remained significant after validation by random permutation. Principal component analysis demonstrated that healthy control urine samples can be differentiated from patients with chronic pancreatitis or acute pancreatitis; chronic pancreatitis patients could not be distinguished from acute pancreatitis patients. This metabolomic investigation demonstrates that this non-invasive technique offers insight into the metabolic states of pancreatitis. Although the identified metabolites cannot conclusively be defined as biomarkers of disease, future studies will validate our findings in larger patient cohorts.