Proteomic analysis reveals molecular mechanism of Cd2+ tolerance in the leaves of halophyte Halogeton glomeratus
Proteomic analysis reveals molecular mechanism of Cd2+ tolerance in the leaves of halophyte Halogeton glomeratus
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DOI：
10.1016/j.jprot.2022.104703
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发表时间：
2022
影响因子：
3.3
通讯作者：
Huajun Wang
中科院分区：
文献类型：
--
作者：
Lirong Yao;Juncheng Wang;Ke Yang;Na Hu;Baochun Li;Yaxiong Meng;Xiaole Ma;Erjing Si;Xunwu Shang;Huajun Wang
Halogeton glomeratus ( H. glomeratus ) is categorized as a halophyte, it can potentially endure not only salt but also heavy metals. The aim of this work was to study the molecular mechanisms underlying the Cd 2+ tolerance of halophyte H. glomeratus seedlings. For that we used a combination of physiological characteristics and data-independent acquisition-based proteomic approaches. The results revealed that the significant changes of physiological characteristics of H. glomeratus occurred under approximately 0.4 mM Cd 2+ condition and that Cd 2+ accumulated in Cd 2+ -treated seedling roots, stems and leaves. At the early stage of Cd 2+ stress, numerous differentially abundant proteins related to “phosphoenolpyruvate carboxylase”, “transmembrane transporters”, and “vacuolar protein sorting-associated protein” took important roles in the response of H. glomeratus to Cd 2+ stress. At the later stage of Cd 2+ stress, some differentially abundant proteins involved in “alcohol-forming fatty acyl-CoA reductase”, “glutathione transferase”, and “abscisic acid receptor” were considered to regulate the adaptation of H. glomeratus exposed to Cd 2+ stress. Finally, we found various detoxification-related differentially abundant proteins related to Cd 2+ stress. These biological processes and regulators synergistically regulated the Cd 2+ tolerance of H. glomeratus . The halophyte, H.glomeratus , has a strong tolerance to salinity, also survives in the heavy metal stress. At present, there are few reports on the comprehensive characterization and identification of Cd 2+ response and adaption related regulators in H.glomeratus. This research focuses on the molecular mechanisms of H. glomeratus tolerance to Cd 2+ stress at proteome levels to uncover the novel insight of the Cd 2+ -related biological processes and potential candidates involved in the response and adaption mechanism. The results will help elucidate the genetic basis of this species' tolerance to Cd 2+ stress and develop application prospect of wild genetic resources to heavy metal phytoremediation. • Halogeton glomeratus ( H. glomeratus ) is categorized as a halophyte, but it has robust resistance to Cd 2+ stress. • A DIA-based proteomic technique was used to investigate the molecular mechanisms for Cd 2+ stress in H. glomeratus. • Numerous biological processes and regulators were related to Cd 2+ stress in H. glomeratus.
