Mechanical Stimulation Mediates Gene Expression in MC3T3 Osteoblastic Cells Differently in 2D and 3D Environments
Mechanical Stimulation Mediates Gene Expression in MC3T3 Osteoblastic Cells Differently in 2D and 3D Environments
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DOI：
10.1115/1.4001162
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发表时间：
2010-04-01
影响因子：
1.7
通讯作者：
Donahue, Seth W.
中科院分区：
文献类型：
--
作者：
Barron, Matthew J.;Tsai, Chung-Jui;Donahue, Seth W.
成功的骨组织工程需要了解三维 (3D) 架构中的细胞活动以及它与二维 (2D) 架构的比较。我们开发了一种灌注培养系统，利用流体流动机械加载细胞接种的 3D 支架。本研究比较了 2D 和 3D 培养物中成骨细胞的基因表达，以及机械负荷对 2D 和 3D 培养物中基因表达的影响。将 MC3T3-E1 成骨细胞样细胞接种到 2D 载玻片和 3D 磷酸钙支架上，静态培养或机械加载流体流。与 2D 静态培养相比，3D 培养中 24 小时和 48 小时 OPN 和 FGF-2 基因表达上调，而胶原 I 基因表达下调。此外，虽然流量在 48 小时时增加了 2D 培养中的 OPN，但在 3D 培养中却降低了 OPN 和 FGF-2。总之，静态条件下 2D 和 3D 成骨细胞培养物的基因表达是不同的。此外，成骨细胞在 2D 和 3D 培养物中对剪切应力的反应不同。我们的结果强调了 3D 力转导研究对于骨组织工程应用的重要性。 [DOI：10.1115/1.4001162]
Successful bone tissue engineering requires the understanding of cellular activity in three-dimensional (3D) architectures and how it compares to two-dimensional (2D) architecture. We developed a perfusion culture system that utilizes fluid flow to mechanically load a cell-seeded 3D scaffold. This study compared the gene expression of osteoblastic cells in 2D and 3D cultures, and the effects of mechanical loading on gene expression in 2D and 3D cultures. MC3T3-E1 osteoblastlike cells were seeded onto 2D glass slides and 3D calcium phosphate scaffolds and cultured statically or mechanically loaded with fluid flow Gene expression of OPN and FGF-2 was upregtdated at 24 h and 48 h in 3D compared with 2D static cultures, while collagen I gene expression was downregulated. In addition, while flow increased OPN in 2D culture at 48 h, it decreased both OPN and FGF-2 in 3D culture. In conclusion, gene expression is different between 2D and 3D osteoblast cultures under static conditions. Additionally, osteoblasts respond to shear stress differently in 2D and 3D cultures. Our results highlight the importance of 3D mechanotransduction studies for bone tissue engineering applications. [DOI: 10.1115/1.4001162]