Optimization of the Cuff Technique for Murine Heart Transplantation
Optimization of the Cuff Technique for Murine Heart Transplantation
复制标题
小鼠心脏移植袖带技术的优化
DOI：
10.3791/61103
复制
发表时间：
2020-06-01
影响因子：
1.2
通讯作者：
Qi, Zhongquan
中科院分区：
文献类型：
--
作者：
Ma, Yunhan;Xie, Baiyi;Qi, Zhongquan
小鼠心脏移植已经进行了40多年。随着显微外科的进步，某些新技术已被用于提高手术效率。在我们的实验室中，我们通过两个主要步骤优化了袖带技术。首先，我们使用内管技术将临时内管插入颈外静脉和颈动脉血管中，以促进血管在袖带上外翻。第二，我们在两位经验丰富的外科医生的合作下完成了完全异位心脏移植。这些改进有效地将手术时间缩短到25分钟，成功率达到95%。在本报告中，我们详细描述了这些程序，并提供了补充视频。我们相信，这一报告的改进袖口技术将提供实际指导小鼠异位心脏移植，并将提高这种小鼠模型的基础研究的实用性。
Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to improve surgical efficiency. In our lab, we have optimized the cuff technique with two major steps. First, we used the inner tube technique to insert a temporary inner tube into the external jugular vein and carotid artery blood vessel to facilitate eversion of the vessel over the cuff. Second, we performed complete heterotopic cardiac transplantation through the collaboration of two experienced surgeons. These modifications effectively reduced the operation time to 25 minutes, with a success rate of 95%. In this report, we describe these procedures in detail and provide a supplemental video. We believe that this report on the improved cuff technique will offer practical guidance for murine heterotopic heart transplantation and will enhance the utility of this mouse model for basic research.