Photo-Controllable Phase Transition of Arylazopyrazole-Conjugated Oligonucleotides
Photo-Controllable Phase Transition of Arylazopyrazole-Conjugated Oligonucleotides
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DOI：
10.1021/acs.bioconjchem.1c00302
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发表时间：
2021-09-09
影响因子：
4.7
通讯作者：
Park, Soyoung
中科院分区：
文献类型：
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作者：
Hidaka, Takuya;Wee, Wen Ann;Park, Soyoung
相变是DNA作为生物聚合物的一个很有前途的方面。阴离子DNA寡核苷酸很容易与聚赖氨酸等阳离子多肽形成络合物，而双链的形成显著影响它们的络合作用和所产生的微隔室。在这项研究中，通过用光调节芳唑并吡唑偶联寡核苷酸的双链形成，系统地诱导了含有DNA和多赖氨酸的微格的相变。我们证明了紫外光照射会破坏DNA双链的稳定性并产生各向同性凝聚体，而可见光照射的双链稳定会导致液晶凝聚体的形成。这种对相变的光控制是高度可重复的，即使在10个周期的光照射后也观察到类似的变化。我们的方法将为定制微型隔室的开发提供一个强大的控制层。
Phase transition is a promising aspect of DNA as biopolymers. Anionic DNA oligonucleotides easily form complexes with cationic polypeptides such as polylysine, and duplex formation significantly influences their complexation and resulting microcompartments. In this study, phase transition of micro-compartments containing DNA and polylysine was systematically induced by modulating duplex formation of arylazopyrazole-conjugated oligonucleotides with light. We demonstrated that UV irradiation destabilized DNA duplex and generated isotropic coacervates, while duplex stabilization by visible light irradiation caused the formation of liquid crystalline coacervates. This photocontrol of phase transition was highly repeatable, and similar changes were observed even after ten cycles of light irradiation. Our approach would provide a robust control layer to the development of tailor-made microcompartments.