Integrated analysis of dysregulated long non-coding RNAs/microRNAs/mRNAs in metastasis of lung adenocarcinoma
Integrated analysis of dysregulated long non-coding RNAs/microRNAs/mRNAs in metastasis of lung adenocarcinoma
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DOI：
10.1186/s12967-018-1732-z
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发表时间：
2018-12-27
影响因子：
7.4
通讯作者：
Zhao, Jie
中科院分区：
文献类型：
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作者：
Li, Lifeng;Peng, Mengle;Zhao, Jie
背景肺腺癌(LUAD)在全球范围内仍是癌症相关死亡的主要原因。本研究从肿瘤基因组图谱数据库中筛选出与LUAD转移相关的差异表达基因(Degs)、miRNAs(Dem)和lncRNAs(Dels)。使用交叉mRNAs进行基因本体论(GO)、京都基因与基因组百科全书(KEGG)途径和共表达网络分析。此外，还对交叉点mRNAs进行了生存分析。结果在LUAD转移和未转移标本中，共检测到1015个DEM、54个DEM和22个DELs。GO和KEGG通路分析已经证明交叉点mRNAs的功能与肿瘤发病机制中的许多重要过程密切相关。在共表达相互作用网络中，共表达网络中有22个基因的度数超过20，这些基因暗示它们与许多其他基因节点有联系。此外，14个靶基因(ARHGAP11A、ASPM、Hells、PRC1、TMPO、ARHGAP30、CD52、IL16、IRF8、P2RY13、PRKCB、PTPRC、SASH3和TRAF3IP3)与LUAD患者的生存显著相关(对数等级P
BackgroundLung adenocarcinoma (LUAD), largely remains a primary cause of cancer-related death worldwide. The molecular mechanisms in LUAD metastasis have not been completely uncovered.MethodsIn this study, we identified differentially expressed genes (DEGs), miRNAs (DEMs) and lncRNAs (DELs) underlying metastasis of LUAD from The Cancer Genome Atlas database. Intersection mRNAs were used to perform gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and co-expression network analysis. In addition, survival analyses of intersection mRNAs were conducted. Finally, intersection mRNAs, miRNAs and lncRNAs were subjected to construct miRNA-mRNA-lncRNA network.ResultsA total of 1015 DEGs, 54 DEMs and 22 DELs were identified in LUAD metastasis and non-metastasis samples. GO and KEGG pathway analysis had proven that the functions of intersection mRNAs were closely related with many important processes in cancer pathogenesis. Among the co-expression interactions network, 22 genes in the co-expression network were over the degree 20. These genes imply that they have connections with many other gene nodes. In addition, 14 target genes (ARHGAP11A, ASPM, HELLS, PRC1, TMPO, ARHGAP30, CD52, IL16, IRF8, P2RY13, PRKCB, PTPRC, SASH3 and TRAF3IP3) were found to be associated with survival in patients with LUAD significantly (log-rank P